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We narrowed to 1,023 results for: NTS

Showing: 661 - 680 of 1023 results

  1. 6 Tips for Grant Writing

    Type
    Blog Post
    ..., or universities may have someone senior from accounts or HR on the judging panel. It’s important for...Skills 5. Practice makes perfect, apply for small grants too There are actually quite a lot of different... researchers, so look into applying for smaller grants from within your institute or a private funding...the experience I gained in writing small travel grants to attend conferences and a seed funding grant ...

  2. Tips for Technical Support Calls

    Type
    Blog Post
    ...companies and organizations selling biological reagents have a ton of data on the catalog page. It’s not.... Although many may not realize it, all of the reagents available at Addgene come from researchers, and...don’t have the resources to recapitulate the experiments performed by our thousands of depositors. That...Google Scholar, which searches scientific articles, patents, and even some doctoral dissertations. Streamline...

  3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ... corrective amounts of protein for any such disease. This technology therefore represents a potential ...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423...doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT (2019) Long-...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...microbiology meeting. Create an outline: Write key bullet points for what you would like to say to help build your...result, and the fundamental takeaway. Writing these points will help you remember what to say and provide ...outline you created for yourself. Hit the key bullet points and elaborate if your audience seems captivated... the project as a whole. In addition to time constraints, there may be particular poster you are interested...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...produced in multiple different cell types, including plants, mammals, and insects. In this post, we will compare...producing virus within 24 to 48 hours. Harvesting timepoints will depend on your specific virus, so this is...methods like ultracentrifugation, while lentivirus supernatants are centrifuged and filtered to eliminate any... important to you — virus type, cost, time, or reagents and equipment. Hopefully this post has provided...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...post-transfection to help remove toxic transfection reagents and keep the cells happy. Harvesting virus Once...widely used method due to the accessibility of instruments in standard labs. When determining titers by ...Adeno-Associated Viral Vectors by Oxidant-Based Disinfectants. Human Gene Therapy, 32(13–14), Article 13–14...sensitivity sufficient to detect single infectious events. Human Gene Therapy, 15(7), Article 7. https://...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...the degron domain is terminal. Between the tag elements (protease and degron) and the protein of interest...other systems reviewed here. The only required components are 1) a SMASh-tagged protein and 2) asunaprevir...reviewed here. The AID system relies on three components. First, the protein of interest must be AID-tagged...SMASh). If you don’t want to deal with additional components beyond the minimum required for a degron system...

  8. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...number of cycles.  Choosing a method  As with all experiments, a number of factors will determine which approach...Resources needed Most molecular biology labs and departments will have qPCR machines available for use. ddPCR...Chung WK. Quantitative analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc... The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... it can amplify DNA using only a short list of reagents and several heating and cooling steps. PCR relies...strand of DNA to add nucleotides to.  Using these reagents and a series of heating (denaturing) and cooling...cycle. However, it is common for dNTPs and other reagents to run low during the final cycles which would...of different molecular biology techniques and experiments. End point PCR’s goal is to amplify a specific...

  10. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...to develop therapies that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing... process can be a bit of a mystery to graduate students in academia. Getting a drug to the market usually...pages. In academia, however, writing papers and grants is an inescapable and essential part of being able...’re often required to at least attempt to write grants. If you don’t enjoy or excel at writing, an industry...

  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...particularly related to the neurosciences.  These developments in microscopy coupled with techniques that make...view of a cleared rat pons, medulla and the first segments of the spinal cord showing the cortico spinal ...spinal tract, CST, spinal cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human...anterior, P= posterior, D= dorsal, V= ventral Developments in light sheet fluorescence microscopy Modern...

  12. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...is the first of what I, scientists, doctors and patients hope are many more clinical successes for gene...the human condition. Luck brought all these key elements together at the right time and place. But it is...limitation, with limited success so far. Luckily for patients, this gene can be targeted to RPE cells using ...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...

  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...require a standard or a reference, meaning saving on reagents (and time)! AAV titering using droplet digital...emulsion technology. Each droplet contains the ingredients for amplification of the target DNA. This partitioning...prepare your sample dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for...digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa, Birei, Teruhide...

  14. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...give to post-docs and graduate students as they complete their appointments in your lab?  Fujii: These days... makes it much easier to knock-in LexA binding elements into the target region. However, it struck me ...Throughout your career, you have held research appointments in the US (New York University School of Medicine...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional flow of information... and memory retrieval. In neuronal tracing experiments, brain tissue is injected with compounds that...injection or iontophoresis (applying small electric currents via inserted electrodes) may be applied, but these...proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been found naturally or have...like these may be a starting point for future developments in the field of AAV vectors. Conclusion An ideal...

  16. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...significant, negative effects in RNA targeting experiments. If Cas7-11 lacked this toxicity, it would represent...forward for CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The... approach to gene replacement therapy for most patients. But how does one go from “cut and paste” to “...Programmable Addition via Site-specific Targeting Elements (PASTE). Finding the right integrase for this ...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...exercise: Consider the issue as if there were no restraints on your problem-solving resources. What would...which people were asked to create a device to help students during an interview. When they were instructed... ideas heard.  So how can we assure that all participants feel comfortable sharing their ideas? And how...have plans in place to be able to continue our experiments. Thinking about these plans before you’ve run...

  18. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...sequencing and bioinformatic technologies are used.  Improvements to sequencing technologies and bioinformatic... lot of effort to stay up-to-date with these improvements. In this post, we discuss the sequencing technologies...addition, DNA synthesis stops. The resulting DNA fragments are pooled and then separated by size using electrophoresis...depositor-provided sequences to confirm the correct plasmid components. For more information on this process, see our...

  19. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...any conference is scientists, from techs to grad students to professors, stopping by to say, “Everything...— in their classroom, in their lab, in their experiments, to enable virtual learning during the pandemic...

  20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...annealing can be affected by the presence of protein contaminants, which is a factor in AAV titration since the...repeating the exact same PCR twice can give titer measurements that vary up to two-fold. How can we get more...low levels of background protein and chemical components, ddPCR may not be an improvement over qPCR (Taylor...). What do Addgene AAV titers mean for your experiments? Because (physical) AAV titers are approximations...
Showing: 661 - 680 of 1023 results