We narrowed to 741 results for: REV

1. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   ...ITAMs ensures effective negative selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun...

2. Simplify Cloning with in vivo Assembly

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   Blog Post

   ...short sequence complementary to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis is performed ...

3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...

4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...within mixed communities and into species not previously cultured or isolated (Rubin et al., 2020).  ...

5. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   Blog Post

   ...across regions. Our investigation of model fit revealed some potential instabilities in the China data...

6. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ... my presentation had only become public in the previous two weeks, which demonstrated to the students ...

7. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...restricted transgene expression in the CNS and PNS Previously to broadly introduce a transgene into a specific...

8. MXS Chaining

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   Blog Post

   ...advantage of being directional, meaning that (in the previous example) one can control whether the third module...

9. Plasmids 101: Colony PCR

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   Blog Post

   ...generalized expected results for the three primers previously described. When using insert-specific primers...

10. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours of valuable time...

11. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ...join together to feast and play lawn games. In previous years, this event was held at Executive Director...

12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ... duty for researchers when considering the peer-review system of journals and grant applications, for ...

13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...strategies such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors ...

14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ...groups to bases at specific nucleotide sequences, preventing the cognate restriction enzyme from cleaving ...

15. Plasmids 101: Shuttle Vectors

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    Blog Post

    ... used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially...

16. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

    ...circumvent sequencing and the need for a DSB in their previous reporter, the Harris Lab created a panel of eGFP...

17. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post

    ... RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled...

18. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ...Holmes, Christina, and William L. Stanford. "Concise review: stem cell antigen‐1: expression, function, and...

19. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.” Chem Biol...

20. Bioinformatics at Addgene

    Type

    Blog Post

    ...rapidly iterate and optimize our pipeline. After reviewing and testing several platforms for engineering ...