We narrowed to 741 results for: REV

1. Plasmids 101: Multicistronic Vectors

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   Blog Post

   ...ITAMs ensures effective negative selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun...

2. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...within mixed communities and into species not previously cultured or isolated (Rubin et al., 2020).  ...

3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   Blog Post

   ...across regions. Our investigation of model fit revealed some potential instabilities in the China data...

4. Simplify Cloning with in vivo Assembly

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   Blog Post

   ...short sequence complementary to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis is performed ...

5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...

6. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ... my presentation had only become public in the previous two weeks, which demonstrated to the students ...

7. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...restricted transgene expression in the CNS and PNS Previously to broadly introduce a transgene into a specific...

8. MXS Chaining

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   Blog Post

   ...advantage of being directional, meaning that (in the previous example) one can control whether the third module...

9. Plasmids 101: Colony PCR

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   Blog Post

   ...generalized expected results for the three primers previously described. When using insert-specific primers...

10. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours of valuable time...

11. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ...join together to feast and play lawn games. In previous years, this event was held at Executive Director...

12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ... duty for researchers when considering the peer-review system of journals and grant applications, for ...

13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...strategies such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors ...

14. Bioinformatics at Addgene

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    Blog Post

    ...rapidly iterate and optimize our pipeline. After reviewing and testing several platforms for engineering ...

15. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...phosphatases that may degrade the target protein. To prevent this, include protease and phosphatase inhibitors...

16. Plasmids 101: Shuttle Vectors

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    Blog Post

    ... used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially...

17. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...deeply interested in pursuing them. We’ve recently revamped our viral vector selection process to standardize...

18. Antibodies 101: The Four ELISAs and When to Use Them

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    Blog Post

    ...set up. Then they block the capture reagent to prevent non-specific interactions. Next comes the binding...

19. Tips for Writing a Good Cover Letter

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    Blog Post

    ... applying to. Be clean and professional. Always review grammar and spelling several times and ask someone...

20. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...Alcaligenes eutrophus, Azotobacter vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio...