We narrowed to 741 results for: REV
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TypeBlog Post...ITAMs ensures effective negative selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun...
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INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...within mixed communities and into species not previously cultured or isolated (Rubin et al., 2020). ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...across regions. Our investigation of model fit revealed some potential instabilities in the China data... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...short sequence complementary to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis is performed ... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post... my presentation had only become public in the previous two weeks, which demonstrated to the students ... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...restricted transgene expression in the CNS and PNS Previously to broadly introduce a transgene into a specific... -
MXS Chaining
TypeBlog Post...advantage of being directional, meaning that (in the previous example) one can control whether the third module... -
Plasmids 101: Colony PCR
TypeBlog Post...generalized expected results for the three primers previously described. When using insert-specific primers... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours of valuable time... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...join together to feast and play lawn games. In previous years, this event was held at Executive Director... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post... duty for researchers when considering the peer-review system of journals and grant applications, for ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...strategies such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors ... -
Bioinformatics at Addgene
TypeBlog Post...rapidly iterate and optimize our pipeline. After reviewing and testing several platforms for engineering ... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...phosphatases that may degrade the target protein. To prevent this, include protease and phosphatase inhibitors... -
Plasmids 101: Shuttle Vectors
TypeBlog Post... used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...deeply interested in pursuing them. We’ve recently revamped our viral vector selection process to standardize... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...set up. Then they block the capture reagent to prevent non-specific interactions. Next comes the binding... -
Tips for Writing a Good Cover Letter
TypeBlog Post... applying to. Be clean and professional. Always review grammar and spelling several times and ask someone... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...Alcaligenes eutrophus, Azotobacter vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio...