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We narrowed to 949 results for: ache

Showing: 661 - 680 of 949 results

  1. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...way through a series of obstacles in pursuit of achieving knowledge. Or, as we in the storytelling business...  Rachel Leeson...

  4. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...fund is an impossibility at our early stage.  After each pitch we made, we considered the feedback and adjusted... well and trust.  This will allow you to discuss each project and business decision before making it, ...

  5. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...Creating a modular, selection-based gene tagging approach Multiple features of C. elegans have made CRISPR...Additional Resources on the Addgene Blog An “elegans” Approach to Better Cas9 Editing Efficiency CRISPR 101: ...

  6. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized region...associated with otherwise more complicated assembly approaches, and opens the door for new opportunities in ...

  7. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat... GFP fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the SunTag sites...

  8. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...over several millennia as their paths cross with each other and with the events of the times."     - Aliyah...non-scientists. It wouldn’t matter whether you had a bachelor’s degree or a PhD, majored in Biochemistry or International...

  9. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...between peak fluorescence and resting fluorescence (achieved through lowering sensor resting fluorescence, ...genetically-defined neuronal populations with intersectional approaches is a growing field that we aim to support by ...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor...hairpin sequences (either MS2, PP7, or boxB) that each recruits a different fluorescent protein (BFP, GFP...

  13. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...drives better after a few drinks. A family member approached me for advice on her baby’s health but adamantly...self-medicate with when they get a cold. A primary school teacher proudly boasted to me that he never eats “anything...

  15. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... communication about experimental methods and approaches. It’s very easy to think that the methods section...increase dramatically. I’m not sure what the best approach to doing that will be, and it will need some effort...

  16. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...combative, field. Of course they are competing with each other, but it's a healthy competition. What an honor...Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts in the Microbiology Community Sign Up ...

  17. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...this question led us to develop a new, rational approach to improve the efficiency of sequence replacement... order of magnitude greater than transfection approaches. Moreover, the cost of reagents is relatively...

  18. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...cells. Positive selection in mammalian cells To achieve stable transfection, there should be a selective...required for efficient selection. This is usually achieved by performing a "kill curve" (basically growing...

  19. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...slides, learn from audiences as they do science outreach and engage with the public, and learn from the...science communication to more graduate students.   Each June, graduate student leaders in science communication...

  20. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...three types of inducible promoters, and examples of each type. Many of these promoter systems are available...promoter. Experimental System Because transcription machinery differs between cell types or organisms, promoters...
Showing: 661 - 680 of 949 results