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... when a good friend, who was a science writer, reached out to me. She was leaving a writing position and...and defining a couple of key terms is a better approach. Also, appealing images and colors draw people...

...thermophiles, bacteria must be grown at lower temps. This approach only works for facultative thermophiles (high ... occurs? This is different than the engineered approach used to make eSpCas9 and SpCas9-HF1. By mining...

...expression of isolated bacterial proteins can be achieved using a bacterial expression system such as the... Susanna Bachle...

...plasmids in a number of different experimental approaches. They first assessed the activation of different...Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH, an open-source biosensor platform...

...focus on the packaging plasmid and take a look at each of its parts. Key elements of the AAV packaging ... domain. Following replication, Rep78/68 stays attached to the AAV genome and docks with the genome onto...

...have been requested more than 100 times each, thereby achieving the coveted “blue flame” status. We’re ...

...step cloning, and overlap extension PCR. Although each type of cloning has its advantages, most scientists..., Meghan Monroy. Meghan Monroy is currently an Outreach Data Curation Intern at Addgene. She is also a...

...Wiles gave us a fascinating and fun look at her outreach work with fluorescent microbes. Check out her ...!). As we dive more into science education and outreach ourselves, we can a learn a lot from these ASM...

...stand clearly and routinely. They are clear with each employee about what they do well and where they ...reading try The Discipline of Teams by Jon R. Katzenbach and Douglas K. Smith or the book on which this...

...analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed...

... Your PI should be the kind of mentor that will teach you how to: interpret data, solve research problems...to ensure the PI will be a good role model and teacher. Do you admire her management style? Does he have...

...Chemogenetics Bryan Roth New serotype pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato AAV8 Molecular tool Massimo Scanziani...and resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the...

...on track to succeed at the same time. I began approaching science funding organizations to request support...all labs sharing materials via Addgene. Perhaps if each of many grantors supporting research could provide...

...began in high school. “I had a great chemistry teacher in 10th grade and then fell in love with science...HMS) where he leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization...

...been extracted from a cell, instead of when it is attached to a cell membrane.  Picking the Right Antibody... Rachel Leeson...

...quantitative assays such as antibody specific activity for each reagent batch. To supplement this, Addgene will ...typically takes a few years for a new project to reach a sufficient number of distributions to cover costs...

...for science’s sake More opportunity to train and teach students Collaborations may be less “complicated...concentrated to business hours (mostly), but can often achieve more structured time management conducive to work...

...way through a series of obstacles in pursuit of achieving knowledge. Or, as we in the storytelling business...  Rachel Leeson...

...control data for each of the materials we distribute. Quality control info can be found on each viral prep’...

...fund is an impossibility at our early stage.  After each pitch we made, we considered the feedback and adjusted... well and trust.  This will allow you to discuss each project and business decision before making it, ...