We narrowed to 716 results for: cas

1. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   ... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

2. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   ...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...impacts the success of BiFC experiments. In either case, overexpression should be avoided because it can...

4. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   ...binding by tweaking those parameters, but in some cases your antibody may just not be suitable for use with...

5. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   ... infect the cell type of interest, which, in the case of Chan et al, was neurons and astrocytes. Why ...

6. Tips for arabidopsis transformation

   Type

   Blog Post

   ... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering...

7. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   ...directly into your preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting...

8. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   ...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing excess...

9. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   ...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

10. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    ...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

11. Plasmids 101: Protein tags

    Type

    Blog Post

    ... blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA...

12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    ... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...

13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    ...used to study protein-protein interactions. In this case, two portions of EGFP are fused to the proteins ...

14. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    ...per 100 ml of starting blood sample, in the best-case scenario [7]. In the clinical setting where patient...

15. Bioinformatics at Addgene

    Type

    Blog Post

    ...Nextflow for bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose ...

16. How to Write a Scientific Review Article

    Type

    Blog Post

    ...Finally, you can sound credible and cool when you casually mention “Haery et al., showed that MYC expression...

17. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ...product or plasmid with an attP donor vector. In this case, we would use PCR to add attB sites to either end...

18. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    ...harbouring a Cre-dependent IL-1 beta overexpression cassette. Expression of Cre in these neurons turns on overexpression...

19. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    ...both homeobox genes that are part of a genetic cascade that determines the cell fate of the dorsal mesoderm...

20. Lab to Office Culture Shock

    Type

    Blog Post

    ...inevitably have downtime and plenty of it. That’s not the case at an office job. I’ve frequently told job candidates...