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...versatile method to identify the expression of transgenes of interest. Furthermore, fluorophore variants...resulted in abundant expression of hM4Di in plasma membranes in both species (bottom panel in Fig. 1). Our ...

... CRISPR having effects on various biological disciplines, the base technology itself is constantly improving...collaboration with the Joung Lab, they used targeted mutagenesis to create HypaCas9 (SpCas9 N692A/M694A/Q695A/...

...time. Searching for literature My go-to search engines for finding scientific literature are Google Scholar...and download what seems interesting or relevant. Harness the power of the Google search engine and sign ...

...you through to a successful outcome and the good ones take this responsibility seriously. As I make my...Vivo Group whose tagline is Bridging Science and Business. Maybe you just need help getting through your...

...Tips for designing your poster Check conference guidelines To begin, you should always follow conference...we’ve just presented some standard formatting guidelines for making a scientific poster, you shouldn’t...

... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

...genomes, scientists can control expression of their genes of interest. While recombinases are powerful gene... knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library...

..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...with other measurements, specifically fluorescent ones? Many of the available biosensors use a single GFP...

...Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different sites...secondary antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity...

...fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency between...diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream of light...

...more I realized that it cuts across multiple disciplines, which really epitomizes what the makerspace ...Caenorhabditis Genetics Center (CGC) at the University of Minnesota has declared our makerspace an educational institution...

...that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing. The more I talked...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...

...can be identified. Even if some mammalian cells lines have similar morphologies, being familiar with these...Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies every 16 hours...

...proteins both transiently or through stable cell lines. Both methods produce high protein yields if transfection...cells)  that allow for continuous expression of genes integrated into the insect cell genome. Both of ...

...and test them in their labs. Figure 2: Guidelines to predict pegRNA efficiencies with pridict.it...any prime editor experimentalist, and especially shines once large-scale prime editing experiments come...

...other colonies containing smaller, recombined backbones that may replicate faster. For this reason, if...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

...CRISPR plasmids categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...

...fewer transcripts of their antibiotic resistance genes and can therefore be cultured in media containing.... Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional...

... the hierarchy of web pages the plasmid page is nested in. You can see in the example above that the bread...Database has more information about common vector backbones.  The backbone modifications listed can refer ...