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... identify the same feature in other plasmids. Aligning sequences with Snapgene    Once we’ve identified...plasmid contains a region with a high amount of guanine and cytosine nucleotides, we might need to send...

..., our antibodies have induced “stabbing” and “poisoning,” but let’s be honest, the elimination style the... as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies...

...bring about change in behaviors and habits in a nonthreatening way. However, I have also found that scientists...interpretation of my feedback to you. What are We Learning: What would have happened if _________? What ...

... precious in less resourced areas of the world.  Nina Dudnik, Founder of Seeding Labs, tells the story...bothered by the fact that I was wasting buffer running my tiny gels in a normal gel box. It took a bit...

...CDF) D Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility...are especially useful to keep in mind if you are planning to purify your plasmid DNA: The insert Bacteria...

...undergo rigorous quality control testing at Addgene meaning they come ready made to accelerate your research...both chemo- and optogenetics. We’re therefore beginning our AAV viral services with these tools. A variety...

...hot springs were known to be diversity hotspots containing abundant biological dark matter and so the lab...leave your samples behind! Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S ...

... which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen. This fluorescent...suited for certain studies. If you have plasmids containing mNeonGreen, mTFP1, or mWasabi that you would ...

...Figure 2). We’ve also created constructs with containing chemical tags such as the SNAP-tag, Halo-tag,...17972876. Kohl, Johannes, et al. "Ultrafast tissue staining with chemical tags." Proceedings of the National...

...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...well.     References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, ...

... controlled with light without the need of any cloning.     What advantages does BLADE have over AraC...lawn on an agar plate, put a mask over the plate containing the pattern of the image, and shined blue light...

...time to make virus! The constructed viral vector containing the cargo of interest is then typically transfected...transduced (like a rogue HEK-293 cell). This virus-containing media can then be placed on whatever cells you...

...Addgene prides itself on being financially self-sustaining through distributions of research materials, ... advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas ...

...] is pretty great for us,” Wang said. “We were planning on and looking at a bunch of different strategies...mutations in the gene may prevent neurons from returning to basal conditions normally after periods of ...

... like to share my own experiences founding and running miLab to describe some key lessons I learned while... upon these people for guidance.  Before even beginning the process of launching a company, you need to...

...stepwise protocol on designing and using RNA scaffolds: Delebecque, C. J. et al. Designing and using RNA scaffolds...

...calcium and other small molecules since the very beginnings of the field. Those engineered sensors were designed... began by isolating a calcium-binding protein, troponin C (TnC), from muscle cells. They identified a ...

...to chat. This can be done randomly or manually assigning which participants to group together. Breakout...doing science, he has assisted many researchers in gaining the confidence to launch new and diverse careers...

...relationships Here are some ways to go about strengthening a new relationship with authenticity and not...(or better yet, become a volunteer). Naturally running into or working with the same people is often the...

...DNA this way. First, start by pouring your gel containing a DNA intercalating dye (ex: ethidium bromide...research Browse protocols for molecular biology, cloning, and more ...