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TypeBlog Post...labs that already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding...microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well...
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Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...check it out!). After months of strategizing, designing, developing, testing - and repeat - we’re proud... a make-over as well. While in the course of redesigning the homepage, and striving to tell our story ... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...large genes, smaller binary vectors with a larger cloning capacity would be very useful: enter pSIM24! To...customized due to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing ... -
9 Tips to Achieve Success in Academia
TypeBlog Post...Tom Ellis, and George Church for answering our burning questions about how they manage and motivate their...Advice for Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...and annotated from more than 45 publications, beginning with the seminal research articles that demonstrated...Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our CRISPR pooled library... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...whole animal models like Drosophila melanogaster, obtaining enough material for a ChIP-seq experiment can ..., the Henikoff lab published a preprint paper outlining improvements to the initial CUT&RUN protocol, ... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post... identify the same feature in other plasmids. Aligning sequences with Snapgene Once we’ve identified...plasmid contains a region with a high amount of guanine and cytosine nucleotides, we might need to send... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...There’s a steep learning curve for Inkscape, but there are lots of resources for learning the software. ... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the cap gene which differ...334 . https://doi.org/10.1007/s40259-017-0234-5 Büning H, Srivastava A (2019) Capsid Modifications for... -
Antibodies 101: Fc Effector Functions
TypeBlog Post..., our antibodies have induced “stabbing” and “poisoning,” but let’s be honest, the elimination style the... as phagocytosis. Other functions We’re still learning about Fc effector functions and all that antibodies... -
Management for Scientists: Giving Feedback
TypeBlog Post...bring about change in behaviors and habits in a nonthreatening way. However, I have also found that scientists...interpretation of my feedback to you. What are We Learning: What would have happened if _________? What ... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post... precious in less resourced areas of the world. Nina Dudnik, Founder of Seeding Labs, tells the story...bothered by the fact that I was wasting buffer running my tiny gels in a normal gel box. It took a bit... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...well. References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post... controlled with light without the need of any cloning. What advantages does BLADE have over AraC...lawn on an agar plate, put a mask over the plate containing the pattern of the image, and shined blue light... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...Addgene prides itself on being financially self-sustaining through distributions of research materials, ... advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas ... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...hot springs were known to be diversity hotspots containing abundant biological dark matter and so the lab...leave your samples behind! Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S ... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post... which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen. This fluorescent...suited for certain studies. If you have plasmids containing mNeonGreen, mTFP1, or mWasabi that you would ... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog Post...Figure 2). We’ve also created constructs with containing chemical tags such as the SNAP-tag, Halo-tag,...17972876. Kohl, Johannes, et al. "Ultrafast tissue staining with chemical tags." Proceedings of the National... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...undergo rigorous quality control testing at Addgene meaning they come ready made to accelerate your research...both chemo- and optogenetics. We’re therefore beginning our AAV viral services with these tools. A variety...