We narrowed to 733 results for: son

1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ... efforts for over 50 years, prefers ligation of Watson-Crick base pair substrates, it demonstrates significant...

2. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   ...cloning. Taking a page from bacterial cloning, we reasoned that we should be able to keep cells moist and...

3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...Y or fourth chromosomes, you are a very unlucky person. Animals are diploid except for their germ cells...

4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   Blog Post

   ...Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu...

5. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...droplets is determined and then analyzed using Poisson statistics to determine the concentration of the...

6. Hot Plasmids: Summer 2025

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   Blog Post

   ...third of the size of Cas9). Figure 2: Comparison of TnpB (A) and Cas9 (B) genome editors. Effector...

7. Hot Plasmids: Winter 2025

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   ...plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song, Y. J., Nguyen, T. H., & Kim, J.-Y. (2024). Optimized...

8. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy...

9. Academic vs. Industry Postdocs

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   Blog Post

   ...is not always the case. There are “difficult personalities” in every work setting and no manager will ever...

10. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ...Cancer – a term familiar to almost every person regardless of profession, race, or gender. Almost no ...

11. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ...of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing low yields...

12. Genome-wide Screening Using CRISPR

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    ...Resources Page. References McDade JR, Waxmonsky NC, Swanson LE, Fan M (2016) Practical Considerations for Using...

13. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...accidental escape to a negligible level. One major reason we chose to publish the concept and likely capabilities...

14. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...talking to people and representing yourself as a real person, so that students see academics as well-rounded...

15. Tips for Using BLAST to Verify Plasmids

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    ... Jason Niehaus...

16. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer...

17. Great Results Start with Great Standard Curves

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    ... ELISAs and When to Use Them A Control for All Seasons More resources on addgene.org Addgene's Antibody...

18. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ...finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where...

19. AAVs in Retinal Gene Therapy

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    Blog Post

    ...gene therapy revolution and the development of personalized medicine. References and further reading 1....

20. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...Medical Center.  You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative...