We narrowed to 734 results for: son

1. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...Y or fourth chromosomes, you are a very unlucky person. Animals are diploid except for their germ cells...

2. Hot Plasmids: Summer 2025

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   Blog Post

   ...third of the size of Cas9). Figure 2: Comparison of TnpB (A) and Cas9 (B) genome editors. Effector...

3. Hot Plasmids: Winter 2025

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   Blog Post

   ...plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song, Y. J., Nguyen, T. H., & Kim, J.-Y. (2024). Optimized...

4. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...droplets is determined and then analyzed using Poisson statistics to determine the concentration of the...

5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy...

6. Academic vs. Industry Postdocs

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   ...is not always the case. There are “difficult personalities” in every work setting and no manager will ever...

7. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   Blog Post

   ...forward the next year when both the Yamanaka and Thomson labs showed the successful generation of human ...

8. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ... efforts for over 50 years, prefers ligation of Watson-Crick base pair substrates, it demonstrates significant...

9. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...Cancer – a term familiar to almost every person regardless of profession, race, or gender. Almost no ...

10. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ...of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing low yields...

11. Genome-wide Screening Using CRISPR

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    ...Resources Page. References McDade JR, Waxmonsky NC, Swanson LE, Fan M (2016) Practical Considerations for Using...

12. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...accidental escape to a negligible level. One major reason we chose to publish the concept and likely capabilities...

13. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ...talking to people and representing yourself as a real person, so that students see academics as well-rounded...

14. Tips for Using BLAST to Verify Plasmids

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    ... Jason Niehaus...

15. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer...

16. Adeno-associated virus (AAV) Guide

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    Guide

    ... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...trans-splicing or overlapping vectors: a quantitative comparison . Molecular Therapy, 4 (4), 383–391. https://...

17. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...Medical Center.  You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative...

18. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target sites from the 18,385...

19. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...lines in B-D represent the pure DNA spectra for comparison. Each of these contaminants will have a slightly...

20. Great Results Start with Great Standard Curves

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    Blog Post

    ... ELISAs and When to Use Them A Control for All Seasons More resources on addgene.org Addgene's Antibody...