We narrowed to 532 results for: abo.3

...yellow fluorescent protein at the time. It is 1.5 to 3 times brighter than the most commonly used GFPs and...We are excited about our new partnership with Allele Biotechnology which allows researchers to deposit...blue-shifted compared to its parent protein CFP484. Collaborating with scientists from Florida State University...mWasabi plasmids here! One of the most notable things about these proteins is that they are monomeric fluorescent... fluorescent protein blog posts Find blog posts about choosing fluorescent proteins for your research ...the Addgene blog Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection...

...forces or (2) the result of competing desires or (3) the presence of obstacles that need to be overcome... your science with others.    My favorite quote about science ever comes from an interview in which someone...storytelling business like to say, science is all about navigating conflict. In literary terms, conflict...find that once I sit down and talk to a scientist about their research, these conflicts come out quite naturally... a simple question, like “Have you ever thought about how X works?”, or even by introducing a person that...devices, we sometimes forget the most important thing about science: It’s cool. Like bowties! Using science ...

... is not prose; it is to be straight to the point. 3. Engage the reader Scientists forget that the reader...successful writer. When I originally talked to my PI about becoming a better writer, I was unsure if I should... a manuscript and critiquing it, were not too laborious, but helped build my understanding of how to generate...and the writing workshop. SWPI's writing workshop About a month later I received another email notifying... manuscript, scientists have a tendency to be elaborate and over explain the material. The point of writing...

...to safely get back to our lab work. Figure 3: When a bison wanders into the campsite, leave your...Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...by flexibly deploying IgnaviCas9 without concern about its stability under harsh conditions. One example...

...last additional experiment requested by Reviewer #3. But why isn’t the plasmid here yet? A quick look ...materials as quickly as possible. (To learn more about why MTAs matter, check out another blog post: Why...provide specific examples if possible Tell them about the Golden Stopwatch! Any organization that deposits...California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute  Fred Hutchinson...

...current with the latest and greatest! Addgene resources 3 Tips to Improve HDR Efficiency for CRISPR Editing ...most popular tweet from the conference is shown above. The blog post I mentioned, John Doench’s How to...forward. To do that, we need to learn much more about cellular DNA repair pathways. An emphasis on cellular...at the Keystone meeting had great things to say about CRISPOR, a tool to help you design, pick, and close...

... Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute of the archaeon...impaired NgAgo activity, with three mismatches abolishing activity. One-guide faithful NgAgo guide loading... how it’s working in the comments section! Read About NgAgo User's First Impressions Here References ... Additional Resources on the Addgene Blog Read About Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants...

...scoop from Addgenie A. Max Juchheim in this post. 3. Plasmids 101: E. coli Strains for Protein Production...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

...International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 More resources on the Addgene blog Viral... using biorender.com   If you’re new to talking about viruses, here’s a quick tip on terminology. “Virus...have a growing number of Viral Vectors 101 posts about each type, so be sure to check our blog, or our ...

...strand completely; it is essential, however, that the 3’ end of the primer corresponds completely to the template...proceed. Usually a guanine or cytosine is used at the 3’ end, and the 5’ end of the primer usually has stretches...stretches of several nucleotides. Also, both of the 3’ ends of the hybridized primers must point toward ...restriction site at the 5’ end of your primer, note that a 3-6 base pair "clamp" should be added upstream in order...amplification process. When designing, if unsure about what nucleotide to put at a certain position within...capabilities. Taking into consideration the information above, primers should generally have the following properties...