We narrowed to 851 results for: Met
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TypeBlog Post...distribution, and we’ve found that this step was sometimes unclear or missed altogether. Emailing Addgene...
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Hot Plasmids - October 2022
TypeBlog Post...through our hot plasmids articles. Think we missed something hot? You can pitch a Hot Plasmids section (antibodies... -
An Addgene Summer Internship
TypeBlog Post...full potential as a scientist. But I expected something wrong. My capstone project was based on plasmid... -
Build Your CRISPR Vocabulary
TypeBlog Post...system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence as the... -
Year of the Camelid: Antibody Style
TypeBlog Post... Genetically modified mice offer an alternative method to producing single-domain antibodies without the... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...cellular cytotoxicity (ADCC) is a slightly more direct method than opsonization. In ADCC, IgG antibodies bind... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...onset. These hits can reveal individual genes or sometimes even genetic pathways that are worth investigating... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...describe all cell lines used in research, using four methods to search out cell lines: Periodic surveying of... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...job descriptions sound interesting? Is the work something you can see yourself enjoying? To find master... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...Addgene's Microbiology Pages Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn how to Network... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...collaborating effectively with other teams. While they sometimes dig in and do some of the work, they must be more... -
Management for Scientists: Giving Feedback
TypeBlog Post...person - intent is usually good. Don’t pretend something went well when it didn’t… but don’t “punish” someone... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...modifying, and improving the CRISPR-Cas9 system. Mehmet Fatih Bolukbasi from Scot Wolfe's lab described... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...in fly culture cells like S2 and carry the metallothionein promoter (e.g. pMT-puro). Worm vectors There... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID: 23524392. PubMed... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...inhibitory neurotransmitter in vertebrates, but methods for directly imaging GABA have been limited. Like... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog Post...genes where there are instances of rare codons, sometimes intentionally modulating expression in ways we... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...cells through fluorescence microscopy or flow cytometry. Using the ACE system, the Harris lab showed that... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post... in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...frameworks and structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672. Inbar, ...