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We narrowed to 1,023 results for: NTS

Showing: 681 - 700 of 1023 results

  1. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...any conference is scientists, from techs to grad students to professors, stopping by to say, “Everything...— in their classroom, in their lab, in their experiments, to enable virtual learning during the pandemic...

  2. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...questions and comments go up early in the day, and the moderators were on top of any comments that were at...Anything”) on r/Science. You can see some of Joanne’s comments on the AMA process below, but we also wanted to...Protocols: Using Apps to Save Time & Track Your Experiments Additional Resources See How We're Sharing ...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    ...biorepositories or have been directly isolated from patients. In these cases, a test known as Short Tandem ... the lower the passage number the better for experiments. This is to ensure the cells are as close to ...original Cell line you purchased, keeping all experiments carried out on low passage cells and this means...and we can progress faster to desired clinical endpoints.   Nick Amiss is an ATCC Outreach Scientist at...

  4. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...have considered myself neutral in any of their disagreements. In fact, conflict resolution isn’t just about...also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive...constructive) conflict. As you perform the experiments to address a reviewer’s concerns, you are making... around missed tasks, stalled projects, and disagreements over prioritization. The source of those conflicts...

  5. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...fun is building within the constraints given. And there were many constraints during the COVID-19 pandemic...talking to their webcams.  Build: I sent the participants interview questions they could answer, and I...remote filming. Learn: There were still a few adjustments I wanted to make to ensure smooth filming in ...

  6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...smaller fragments of the transgene have been seen with the hybrid method. While these fragments are likely...co-infected with both part A and part B AAVs, the fragments reassemble and produce the full-length gene. Below... donors for two consecutive CRISPR-mediated HDR events which results in the integration of a larger donor...

  7. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if...target time frame.   I suggest that all graduate students and postdocs form peer mentoring groups to practice... and data professionally). Do remember the fine points of visual aids. Here’s a great slideshare with ...sharing model works. See if you can elicit helpful comments from your audience as in “Does anyone else have...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...about are (1) your experimental conditions and measurements; (2) the cells you’re targeting; (3) how much...and then test each vector before starting your experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger..., at an adequate level for your experimental measurements. The number itself is determined through validation...  Many other factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors...

  9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...months. With CRISPR (B), you can inject CRISPR components directly into a zygote and obtain a knockout ...complexes of Cas9 protein and the separated guide RNA components (crRNA and tracrRNA) for maximum Easi-CRISPR ...gRNAs. As with other homology-directed repair experiments, you’ll want the guide cut site to be as close... simple method for delivery of genome editing components to the germline. In contrast to standard microinjection...

  10. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...parallel between the timelines of the scientific advancements and the noteworthy work of fiction that it inspired...genetics was booming, SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke, James Blish... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...

  11. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    .../error, variance, data ranges. how closely data points fall to each other (see Fig. 1). Alpha values...certain information about. The assumptions and requirements that need to be met for a parametric test are... - Quantitative If your data doesn’t fit the requirements above, a non-parametric test may be the way ... were additive, etc.). ANCOVA and MANCOVA All variants of ANOVAs discussed so far assume that the independent...

  12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...are often little tricks that are essential for experiments but that nobody talks about. After months of ...A549 cells to express Cas9. In these particular experiments, we generated A549 cells stably expressing Cas9...good gel image. If you are analyzing short DNA fragments, run PAGE instead of agarose to get sharper bands... quick and easy it is to share information and regents, it’s exciting to think that no matter who you ...

  13. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    ...AAV experiments to the Data Hub. Submit your data by Nov. 12 to compete. The top ten participants will...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...

  14. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...capture in these types of samples is their fast movements. Depending on the sample, a temporal resolution...millisecond scale may be needed. Most commonly these experiments are performed on a fluorescent widefield microscope...your institution when heading into a new set of experiments. Many thanks to our guest blogger, Doug Richardson...Choose the Brightest Fluorescent Protein for Your Experiments Resources on Addgene.org Browse Our Fluorescent...

  16. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ...scientific communities and welcome suggestions in the comments.  X has moved significantly away from the functionality... Every community benefits from a diversity of viewpoints, and we acknowledge there may be a variety of...invite you to follow us across our social media accounts.  -Addgene   ...

  17. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ... Lab tip #2: Tube flick trick When combining reagents in the lab, it is important to mix them thoroughly...before use.  For efficient mixing, add the various reagents to a single tube and simply flick the tube several...everything Use barcodes and scanners to keep track of reagents in your lab. With barcode tracking, you will always...

  18. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...removed the uracil glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil ...developed two fusions of a CBE with other BER components: the DNA repair protein rXRCC1, or the DNA binding...both E. coli and C. elegans could be effective components of CGBEs. Their eOPTI-CGBE and cOPTI-CGBE had...they expanded CGBE target scope by creating BE variants with other Cas9s that recognize different PAMs...

  19. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...Tom McFadden, recently worked with high school students in the bay area to create a plasmid rap video ...has made many more Science rap videos to teach students around the globe and is pushing SciComm further...DNA. 2. The Immortal Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the African...

  20. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...AAV packaging experiments. Let us know what you think about the calculator in the comments section below... do the math for you and it’s simple to make adjustments for the type of tissue culture dish and number...
Showing: 681 - 700 of 1023 results