Addgene

We narrowed to 710 results for: REV

Showing: 681 - 700 of 710 results

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PiggyBac-ing Through the Genome Editing Field

Type

Blog Post

...disrupt the target site for the nuclease thereby preventing re-cutting. Transposase removal of the cassette...
Finding Your Perfect Job After University

Type

Blog Post

...work-life balance compared to working in research. My previous experience working in Industry was really valuable...
Building Global Connections with the International Mentorship Program USA-EUROPE

Type

Blog Post

...responses to the mid and post-program self-assessment revealed that their knowledge and skills had improved significantly...
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

... strategies in C. elegans As brilliantly and irreverently illustrated in the parable of the Geneticist...
Transferable Skills Guide: Teamwork

Type

Blog Post

..., and time to the cause ever since. Unlike the previous two team types, the skills are not cherry-picked...
Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

Type

Protocol

...precautions, tuck your sleeves into your gloves to prevent potential spills down your sleeve. This may or ...
With an Eye Towards the Future, We Look Back at the March for Science

Type

Blog Post

...Additional Resources on the Addgene Blog Read Our Previow Post on the March for Science Learn How Future ...
Protocol - How to Streak a Plate

Type

Protocol

...each new section crosses at least one line of the previous section so that it will contain some bacteria....
Plasmid Modification by Annealed Oligo Cloning (with Protocols)

Type

Protocol

... MfeI - CAATTG ). The bottom oligo will be the reverse compliment so that they can anneal. Top oligo: ...
CRISPR 101: Multiplex Expression of gRNAs

Type

Blog Post

...sequences. You also have the option of including a previously-designed tyr gRNA, which causes hypopigmentation...
Centrifugation

Type

Protocol

...given as Relative Centrifugal Force (RCF) or Revolutions Per Minute (RPM), these are not the same units...
Molecular Biology Protocol - Restriction Digest of Plasmid DNA

Type

Protocol

... vector, you will need to use a phosphatase to prevent re-circularization of the vector if you are cloning...
Immunocytochemistry

Type

Protocol

...antibody concentration will vary between antibodies. Review the manufacturer's instructions before starting...
AAV Purification by Iodixanol Gradient Ultracentrifugation

Type

Protocol

...hgtb.2015.051 (Link opens in a new window) . Last reviewed on: November 7, 2023...
Protocol - How to Run an Agarose Gel

Type

Protocol

...well, maintain positive pressure on the sample to prevent bubbles or buffer from entering the tip. Place ...
Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

Type

Protocol

...digestion, you will need to use a phosphatase to prevent re-circularization of the recipient plasmid. You...
Western Blot

Type

Protocol

...and blocking buffer will vary between antibodies. Review the instructions before starting your experiment...
Antibody Validation Using the Indirect ELISA Method

Type

Protocol

...against the micrograms of antigen loaded. Last reviewed on: October 8, 2024...
Technical Design of a Western Blot

Type

Blog Post

...performing your own validation assays. If you’re using previously published antibodies, check the manuscript and...
Isolating a Monoclonal Cell Population by Limiting Dilution

Type

Protocol

...11(8):783-4. (Link opens in a new window) Last reviewed on: September 9, 2023...

Showing: 681 - 700 of 710 results

Results per page:

PiggyBac-ing Through the Genome Editing Field

Finding Your Perfect Job After University

Building Global Connections with the International Mentorship Program USA-EUROPE

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Transferable Skills Guide: Teamwork

Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

With an Eye Towards the Future, We Look Back at the March for Science

Protocol - How to Streak a Plate

Plasmid Modification by Annealed Oligo Cloning (with Protocols)

CRISPR 101: Multiplex Expression of gRNAs

Centrifugation

Molecular Biology Protocol - Restriction Digest of Plasmid DNA

Immunocytochemistry

AAV Purification by Iodixanol Gradient Ultracentrifugation

Protocol - How to Run an Agarose Gel

Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

Western Blot

Antibody Validation Using the Indirect ELISA Method

Technical Design of a Western Blot

Isolating a Monoclonal Cell Population by Limiting Dilution

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