Antibodies 101: Immunoprecipitation
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Blog Post
...phosphatases that may degrade the target protein. To prevent this, include protease and phosphatase inhibitors...
We narrowed to 741 results for: REV
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Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...deeply interested in pursuing them. We’ve recently revamped our viral vector selection process to standardize... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...set up. Then they block the capture reagent to prevent non-specific interactions. Next comes the binding... -
Tips for Writing a Good Cover Letter
TypeBlog Post... applying to. Be clean and professional. Always review grammar and spelling several times and ask someone... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...Alcaligenes eutrophus, Azotobacter vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post... RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...Dr. Amanda L. Gunn and Dr. Denise M. Akob for reviewing and commenting. Binning Singletons is especially... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...journal article, find blogs about the study, or even review Facebook and Twitter comments. On We Share science... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post... Labor to update the FLSA, which had not seen revisions since 2004. In July 2015, the Department of Labor... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...fluorescent protein fusions in live cells in real time. Previously, we have described a number of practical considerations... -
Plasmids 101: Control Plasmids
TypeBlog Post...aliquot/preparation of the test plasmid. Let us now revisit our experiment. In Figure 1, it appeared as though... -
Tips for Using FRET in Your Experiments
TypeBlog Post... Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes and... -
March for Science
TypeBlog Post...hypothesis. Looking at the data, scientists then revise their hypotheses and the cycle begins again. No... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...deficient in dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...Biotinylation Other resources Proximity labeling review: Trinkle-Mulcahy, L. (2019). Recent advances in... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...and Samie R. Jaffrey. "A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog Post...mammalian cells appeared in 2013 (Cong et al., 2013; reviewed in Lander, 2016). The first base editor was reported... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...structure, function, and regulation. Biophysical Reviews, 13(6), 1081–1090. https://doi.org/10.1007/s12551... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post... visualized, achieved through, for example, a previously induced gene knockout. Besides amine- and DNA-binding... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits ...