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Addgene

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We narrowed to 949 results for: ache

Showing: 681 - 700 of 949 results

  2. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...tracking the reaction progress as in real time PCR, each reaction partition is individually assessed for ...The amount of both mutated and wild type target in each sample can be quickly calculated (details in [2]...

  4. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...other ways to choose restriction enzymes for this approach. If you’re planning to go this route, check out...helpful in verifying mutations that wouldn't be reached by sequencing from either terminus. Want to know...

  5. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...replicate episomally, essentially remaining an extrachromosomal part of the host genome that can replicate...j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...

  6. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...usable product with two 5′ overhangs, so these approaches require higher concentrations of insert to succeed...multicomponent assembly have 5′ or 3′ sequence homology to each other, they may be assembled incorrectly. To overcome...

  8. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...conventional breeding; synthetic biology is required to achieve this novel and unique phenotype. It’s an unexpected...flowers, bugs, and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from ...

  10. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...important to establish the type of audience you want to reach with your paper. Is it the wider scientific community... and the entire referee report are permanently attached to the published article. In addition, authors...

  11. Transfection for Recombinant Antibodies

    Type
    Protocol
    .... Wipe down all pipettes and equipment with 10% bleach prior to use. Reagent Preparation 100 mM Valproic...containing 108 mL cells: Transfer 6 mL of BCD TFX into each of two 50 mL tubes. Cap the tubes and incubate for...

  12. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...if desired, but it's not required for their use. Each RANbody is detectable by two different tags: a reporter...particular applications. Read on to learn more about each reporter or check out table 1 for a summary. HRP...

  13. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...

  14. Summer SciComm: Preprints

    Type
    Blog Post
    ...There are lots of ways scientists communicate with each other: from formal mechanisms like publishing a ...potential collaborators, blogging and more. These each have different goals, formats, and processes, and...

  16. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...ice for 5 min. Add 150 μL of cold Solution III to each tube. Mix by inverting several times. A white precipitate...careful, the pellet is harder to see and less well attached to the tube after the 70% ethanol wash. You can...

  17. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...infect more HEK293 cells and produce more rAdV to reach higher titers. Successfully infected cells will ...at increasing scale over the course of 1-2 weeks. Each round of amplification should result in a 10-100...

  20. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...about the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update ...
Showing: 681 - 700 of 949 results