We narrowed to 716 results for: cas
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TypeBlog Post...Nextflow for bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose ...
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pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...per 100 ml of starting blood sample, in the best-case scenario [7]. In the clinical setting where patient... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post...outside of the box (most likely your lab, in this case). It is important to realize that you have to take... -
Lab to Office Culture Shock
TypeBlog Post...inevitably have downtime and plenty of it. That’s not the case at an office job. I’ve frequently told job candidates... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post... by looking at the different populations. In the case of Figure 2, one population is B- and another two... -
Hot Plasmids - November 2023
TypeBlog Post...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies... -
Viral Production at Addgene
TypeBlog Post... contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event of contamination... -
How to Write a Scientific Review Article
TypeBlog Post...Finally, you can sound credible and cool when you casually mention “Haery et al., showed that MYC expression... -
Plasmids 101: Gateway Cloning
TypeBlog Post...product or plasmid with an attP donor vector. In this case, we would use PCR to add attB sites to either end... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...even make their own antibiotic compounds. In such a case, the microbe itself would be unharmed by the compound... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...the salary changes expected for postdocs in many cases should be as described in the notice of the new ... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...in a higher volume of lower titer virus. In such cases you may want to consider concentrating your viral... -
Tips for Using FRET in Your Experiments
TypeBlog Post...with the laser used to excite the donor. In that case the cross-talk is high and your background signal... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...oftentimes put to therapeutic uses, and in those cases it's important to identify the right tissue-specific... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...and migration, initiating intracellular signal cascades, and allowing agile responses to mechanical forces... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...perceive any conflict as inherently bad, and in many cases it can be. If people or teams can’t see eye to eye... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post... same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...cellular RNA to specific intracellular locations using Cas9 and track the localization of the complex. Broccoli... -
March for Science
TypeBlog Post...Information on how to contact your representatives in case you would like to talk with them about science: ... -
Evolution of Lab Techniques
TypeBlog Post...detection methods are also commonly used. In all cases, the result can be quantified by the strength of...