We narrowed to 830 results for: eed

1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   Blog Post

   ...Your gRNA Find the Ideal CRISPR Software for Your Needs Resources on Addgene.org Catch up on Your CRISPR...

2. Components of CRISPR/Cas9

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   Blog Post

   ...CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 to their...

3. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ... best choice, the fluorescent protein properties need to be scrutinized under relevant conditions. The...

4. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ..., reversible activation of AgRP neurons drives feeding behavior in mice. J Clin Invest. 2011 Apr;121(4...

5. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...methylation status at a double-strand break that needs repairing. They may be looking at chromatin at the...

6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   ...characteristic of the Cas13 superfamily. Yan et al. proceeded to study orthologs from Eubacterium siraeum (EsCas13d...

7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ...successful CRISPR editing experiments, there was a real need in the scientific community for efficient real-time...

8. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ...regardless of outcome, and are able to receive feedback on their study plans at a point in the research...

9. Building and Validating Recombinant Antibodies: A Case Study

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   Blog Post

   ... This greatly limited the number of clones that needed to be screened through functional assays. Following...

10. Antibodies 101: Monoclonal Antibodies

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    Blog Post

    ...you make the best decision for your experimental needs.     References and resources References Bradbury...

11. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    Blog Post

    ...cell. Microinjection requires the use of a fine needle to deliver nucleic acids to individual cells. Electroporation...

12. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

13. Early Career Researcher Toolbox: Social Media for Scientists

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    Blog Post

    ...are using social media professionally, and 2) I needed to start using Twitter. Sharing Chemistry Cat...

14. OCaMP: A New Calcium Indicator for Neural Imaging

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    Blog Post

    ... in the brain. For this to work, each indicator needs to emit a different color of light and be compatible...

15. Fluorescent Proteins 101: Luciferases

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    Blog Post

    ..., which emits light. No excitation illumination needed — just the luminescent substrate. This approach...

16. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ...CRISPR nucleases for many more specific research needs. In this update, we will focus on the most recent...

17. A Deep Dive into BioBuilder

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    Blog Post

    ... workshops, and customized programs to meet the needs of students and teachers. It’s possible to join ...

18. Plasmids 101: Blue-white Screening

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    Blog Post

    ...plates: X-gal is light and temperature sensitive and needs to be added to media after autoclaving. If spread...

19. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...

20. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ... common to biological sources of DSBs, and which need to be processed. Processing occurs prior to ligation...