... outcomes. The quality of the work is often dramatically improved when tasks are distributed appropriately...team manager must then facilitate appropriate communication between members of the team to ensure that no...of the overall project gets left undone or is duplicated. With each component of the project completed.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...clear outcomes in mind, you won’t be able to communicate these well to the task assignee and this is a...with most managerial responsibilities, good communication is at the heart of delegating for success.  ...

...plasmids can be used to carry out any biochemical and biophysical study involving protein stability or ...degradation. Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... most scientists use mutant animals that are genetically altered to overexpress endogenous RNAi pathways...created an improved RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols...facilitate protein-protein interactions and mediate biochemical processes. One of the largest and best characterized...

... our knowledge. Disease Applications Q13: Does AAV have potential applications in diseases which may be...1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011 - 1x1012 vg/...Have you or others been able to successfully systemically transduce and then retransduce a model organism...as multiple sclerosis?  A: Basic science and preclinical researchers have used the flexibility of AAV ...CRISPR tools is an exciting area for future AAV application to epigenetic editing. This area is discussed...

...cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this...antibody, meaning that the antibodies are largely identical between lots.  Disadvantages of monoclonal antibodies...there will be changes between lots over time. Specifically, the antigen-binding sites of the antibody -... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:...2016) Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007. https://doi.org/10.1074... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

...Stupid, overwhelmed, discouraged, tired…words that indicated there were many barriers between our audience ...readers.    Figure 1: An image from AAV Purification by Iodixanol Gradient Ultracentrifugation ...Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient Ultracentrifugation. The... reader accessing the information? This can be physical or digital. For instance, you would present information...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

... useful information on scientific literature, technical skills, and experiences from thousands of laboratories...antibody and content team, we identified what applications should be included and what basic information... The guide was then assigned to our science communication specialist, Rachel, to write. Identify expertise...through an editing process and final check before publication.   Do research! Nearly everything we write entails...Guide figures. This requires a fair amount of communication between our content team and our graphic designer...graphic designer upon request that the science communicator "just sketch a western blot out quickly." Final...

...getting to talk to so many different people! I communicate with customers, software developers, as well ...from interacting with others, and have strong communication skills to tailor your message to any type of... to solve is clear, but the solution is more complicated. You’ll need to be creative to be able to work...m always catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss ...automated protocols. 12:00 PM--Catch up. Finish any communications with my UK team members before they log off... at Oxford Nanopore Technologies for the North America region. She is particularly interested in DNA sequencing...

...problems. Rapidly dividing cells initiate genome replication more than once per cell division and thus, genes...genes closer to the origin of replication will be overrepresented compared to those further away. As a...cells are dividing rapidly, multiple rounds of replication are initiated before the cell has divided. This...maximal gene expression near genomic origins of replication, the stabilized promoters created by Segall-Shapiro...the control of IPTG. Because RepA is a plasmid replication initiator protein, they could alter copy number...dosage in E. coli using DIAL strains." Journal of biological engineering 5.1 (2011): 10. PubMed PMID: 21787416...

...editing performance at therapeutically relevant gene targets and expand the application to the possible treatment...AsCas12f1. They found that this system, without any modifications, is effective in genome editing in both bacteria...ribonucleoprotein, and AAV.  Read the paper in Nature Chemical Biology Find the AsCas12f1 plasmids   Selective... Mike Lacy The retroviral-like protein PEG10 specifically binds its own mRNA to facilitate its packaging... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...

...days prior to the implementation date, our data indicated that, while 69% of all postdocs were expected ... department at the University of Massachusetts Medical School. We are working with postdocs at other institutions...sent, with the lack of transparency and poor communication of their plans (many postdocs - and in some ...to reach out to institutions with a statement indicating that we encourage them to keep raising salaries... December 1st. Salary inconsistencies across geographical regions When we originally started gathering...changes in science. Follow him on Twitter @BiophysicalFrog and Future of Research @FORsymp.   Additional...

... audience and convey your ideas clearly and strategically is powerful in almost any career. It allows ...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm... the open windows in the middle of your talk. Technical difficulties turn your slide deck into a chalk...everyone! Everybody makes mistakes, encounters technical difficulties, and runs into unexpected stumbling..., to a group of peers who are there to provide critical feedback, often with a semi-international audience...your own native language. And, at least in the biomedical field, with very little text on the slides and...

...composed of four peptide chains (two identical heavy chains and two identical light chains) each with a constant...antibodies means that it is relatively easy to physically divide up the different regions, both through... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...fragments described above can be used for similar applications and have similar advantages, though with some...David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody Fragments. Antibodies 8(...

...and on self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory...receptors (NRs), their coregulators, and their physiological endocrine ligands. Nuclear receptors and their...murine members. NRs permit the integration and communication of such signals between central and peripheral...circadian regulation, as well as in important physiological states such as adipogenesis and macrophage activation... activation. Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa cells...widely used today.  The sheer abundance of these critical research tools is a double-edged sword, however...

.... When an ELISA kit is ordered, the wells are typically already coated with the antibody. Then, you add...process is the addition of a detection antibody (typically a monoclonal antibody) that also recognizes the...the most common type of competitive ELISA is a modification of the sandwich ELISA as described here, the...ELISA, the only difference being the additional amplification step, which helps to visualize low-abundance...concentrations of your protein of interest. The optical density (OD) - the measure of light absorbed by...concentration of any experimental wells in that range of optical densities. If your standard curve does not have...

... for PAM-less editing   Guides for CRISPR are typically selected by the ‘quality’ of the gRNA sequence...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...the Liu lab through phage-assisted evolution, specifically with the goal of generating Cas enzymes with...compatibility. This alternative PAM requirement adds a significant amount of flexibility compared to a strict single-compatibility...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...increases the flexibility of gRNA placement significantly over SpCas9 and more so than Cas9s with an NG...

...prevent the formation of replication competent lentivirus (RCL). Replication competence can arise from...end-users, size of the transgene, and downstream applications should all be considered when choosing the correct...unconcentrated prep. For example, at Addgene we typically concentrate 100-fold meaning that a 10 cm2 dish... as resources available, cost and downstream applications. For example, while the PEG precipitation method...lentivirus is the cell line that is used for titering. Typically researchers will choose a relatively permissive...

...targeting anywhere in an exon. But things get more complicated if you’d like to use homology directed repair...edits or knock-ins. Editing efficiency decreases drastically once your cut site is more than 10 bp away from...They created a selection phage (SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase...loci displayed a broad range of PAM sequences, indicating they may be less predictable than SpCas9 off-... that we have only scratched the surface of modifications that can be made to Cas9. It is a pleasant surprise...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

...Gonçalves and his colleagues at Leiden University Medical Center are now available at Addgene along with ...and very efficient introduction of DNA into therapeutically relevant, non-transformed mammalian cells. ...and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral vectors Since...and used AdV vectors to edit genes in several biological systems. It has been used for instance in inherited...Strategies for Tissue-Specific Targeting. In: Applications of viruses for cancer therapy. Elsevier, pp ...Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 ....

...experts in science communication in real time! I follow great online science communicators like Phil Plait...nontraditional” career path long ago. Use your analytical mind to test and discover what career suits you...about any topic I like and gain experience in communication, editing, marketing, and website organization.... She is particularly interested in science communication and fundamental molecular biology.      Additional...  science communication...

... familial aspect of Addgene. As our size has drastically increased, it would have been a tight squeeze...event! Addgene company culture At Addgene we organically developed the philosophy of  “work hard, play...department) right in your breakroom. 5. Throw some physical activity into the mix It’s easy to sit around ...be an obvious one, but don’t make things too complicated for yourself (especially if you are the sole ...sole event planner)! If you have a very intricate itinerary, there is a higher likelihood that something ...Delegate- it will save you time and sanity!    10. Communicate Make sure people know that there is something...