Viral Vectors 101: Pseudotyping
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Figure 1: Lentiviral production uses three plasmids: (1) The transfer plasmid, (2) the envelope plasmid...
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Getting the Most from Your Lentiviral Transduction
TypeBlog Post...after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy is... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more accessible... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...introduced in vivo either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...Ribonucleoproteins Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages Find Validated... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...the Packaging Capacity of Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog Post...Clinic Resources on Addgene.org Browse CRISPR plasmids for base editing Read our CRISPR 101 eBook Brush... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...fight. But you also can’t be the type of person who avoids conflict at all costs or actively runs away from... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...Western Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post... readily accessible recombinant antibodies and plasmids. To encourage collaboration and education in protein... -
Evolution of Lab Techniques
TypeBlog Post...Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...as a source of sequenced and quality controlled plasmids and viral vectors. When working with a plasmid... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...can be a real challenge. On top of that, in the midst of a rapidly changing, globalized environment re-emerging... -
Important Considerations When Using AAVs
TypeBlog Post...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post...viruses, but you can find a list of validated gRNA plasmids on our website. There are a few reasons to target... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...code for the same amino acid) for various amino acids. Essentially, instead of forcing sequence diversity... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...handling glassware with brightly colored hazardous liquids. Working in a lab is nothing like a typical part-time... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post... Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...Check out Our Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent ...