Skip to main content
Addgene
Showing: 681 - 700 of 928 results

  1. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...“Never Leave an Empty Seat” at conferences and seminars, as this is a valuable time to network Attend...

  3. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...be sufficient to return the sample back to the original pH. What about putting it back in the freezer...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods...

  4. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed ... Research Methods. We also pulled the big interdisciplinary journals: Nature, Science, PLOS One, and PNAS...

  6. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...

  10. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...have 4 hours and 35 students! After a course coordination meeting with some other professors, I went home...students had to pick the best strategy and propose a final gene therapy approach for the disease to be openly...

  11. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...restriction enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New...typical two oligos, is more cost effective and eliminates the annealing step usually used to create a double-stranded...

  12. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...the actions of a single person but through the coordinated efforts of many individuals. Check out Joanne's... a project progresses it is easy to forgot the original plan. Write down your plans in a shared document...

  13. Hot Plasmids - February 2022

    Type
    Blog Post
    ...identifying guide selection rules, and reducing recombination by finding acceptable variations in DR sequences... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...

  14. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...dissociation from the GPCR allows for binding of a new inactive heterotrimeric G protein complex and subsequently...pathways from ligand to receptor to G-protein, and finally, to a response? Let’s take a look at a few examples...

  15. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... very similar to the widely used FRET, but it eliminates the need for an excitation light source. Instead... medium to allow RLuc8 to produce photons. The original nano-lantern emitted yellow-green light and exhibited...

  16. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...from Bryan Roth’s lab at the University of North Carolina, Chapel Hill. These can be used to investigate... only be expressed in cells that express cre recombinase. Typically, this feature is used to attain cell-specific...

  18. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...applications Vaccine development Attenuated and inactivated viruses pioneered vaccine development well over...

  19. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward across...for multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons ...
Showing: 681 - 700 of 928 results