We narrowed to 903 results for: nes
-
TypeBlog Post...protocols and examples of applications from across disciplines now available. In this post, we’ll review the...example, cytoskeletal structures that form continuous lines in pre-expanded samples can sometimes take on a ...
-
MXS Chaining
TypeBlog Post...derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin ... might not, however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...levels. BiFC quantification This is where BiFC outshines FRET — BiFC experiments only require that you ...This is straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...Japan, of course, it is optimized for living for Japanese, which is not trivial, and the grant situation...eager to interact, which I don't think is good for Japanese science. I really miss the intellectual environment... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...Further Reading Quiñones-Valles, C., Espíndola-Serna, L., Martínez-Antonio, A., Quiñones-Valles, C., Espíndola-Serna... -
Tips for Writing a Good Cover Letter
TypeBlog Post...Other fields, often highly technical or skills-based ones, may not use them very often. Similarly, entry-level... cover letter should be no more than a page. Conciseness is key! If possible, address to the professor... -
An Inside Look into Addgene's Dev Team
TypeBlog Post... than optimal user experience and the overall soundness of the software design. Each of us brings slightly...Demo details for this would be something along the lines of showing that the checkout takes the user directly... -
Career Insights: Technical Support Specialist
TypeBlog Post...easier. You should also demonstrate a strong willingness to help others with their problems, a general...sorts of details about the application, just not the ones that matter. Therefore, if there is a bit of a Sherlock... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...base pairs long and found upstream of their target genes. The sequence of the promoter region controls the...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...the scientists and scientific process behind the scenes to the new antibodies and research coming out of... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...illumination, substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear.... Build: If you’ve ever watched any behind the scenes footage in the last several years, you can usually... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...dissociate from the ligase and bind to exposed lysines on neighboring proteins. Thus to capture protein...monitor protein-protein interactions Split-BioID combines a protein fragment complementation (PCA) technique... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...need to keep paper records as well as electronic ones? It is important to know in which ways your data...functionalities such as: electronic signatures, electronic witnessing and audit trails. How does the ELN provider ... -
10 Steps to a Perfect Science Talk
TypeBlog Post...talks for non-academic settings. Many of these guidelines will apply to any type of science presentation...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...a challenge. Enter Easi-CRISPR: a method that harnesses the power of ssDNA donor molecules for homology...mouse embryonic stem cells. After selecting for clones with the recombined product, you’d microinject ... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...skills, positive disposition and motivation, open mindedness, and the ability to accept feedback and adapt...receive immense personal gratification as they witness their student’s progress and increases in self ... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine amino acid...the fluorophore’s microenvironment to enhance brightness, stability, or other properties is an active ... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...Statistics – The basics Statistical analysis is what determines if there is statistical significance among data...There are many, many statistical tests beyond the ones detailed in this article. These are just some of...