We narrowed to 985 results for: nin
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TypeBlog Post...time to make virus! The constructed viral vector containing the cargo of interest is then typically transfected...transduced (like a rogue HEK-293 cell). This virus-containing media can then be placed on whatever cells you...
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Plasmids 101: Origin of Replication
TypeBlog Post...CDF) D Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility...are especially useful to keep in mind if you are planning to purify your plasmid DNA: The insert Bacteria... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...] is pretty great for us,” Wang said. “We were planning on and looking at a bunch of different strategies...mutations in the gene may prevent neurons from returning to basal conditions normally after periods of ... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post... like to share my own experiences founding and running miLab to describe some key lessons I learned while... upon these people for guidance. Before even beginning the process of launching a company, you need to... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...stepwise protocol on designing and using RNA scaffolds: Delebecque, C. J. et al. Designing and using RNA scaffolds... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...calcium and other small molecules since the very beginnings of the field. Those engineered sensors were designed... began by isolating a calcium-binding protein, troponin C (TnC), from muscle cells. They identified a ... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog Post...DNA this way. First, start by pouring your gel containing a DNA intercalating dye (ex: ethidium bromide...research Browse protocols for molecular biology, cloning, and more ... -
25 Work From Home Tips for Scientists
TypeBlog Post...while listening to podcasts made for kids. Plan out meals and snacks ahead of time. Find learning activities... -
Cultivating Community Science at BosLab
TypeBlog Post...laboratory for citizen scientists. In addition to maintaining a laboratory to support both individual and community...that everyone gets proper equipment and safety training prior to getting started. For example, Timothy... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...PhD student at MIT. While I had always enjoyed learning about biology in high school, it wasn’t until ...experiences and knowledge with each other to help make learning biology more fun – bit by bit. Ally Huang is ... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use... lowering sensor resting fluorescence, while maintaining high peak fluorescence) and is useful for imaging... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...datasets. This study demonstrates the power of combining the analysis of publicly available metagenomic...datasets with biophysical studies and the potential of mining datasets for research and innovation. Find MerMAIDs... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use... for interpreting the results of studies. When planning AAV experiments, scientists often have to choose... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...retina. This pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. ...Central PMCID: PMC5026494. Zhang, Qiang, et al. "Designing a green fluorogenic protease reporter by flipping... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post...remembered kindly. However, despite many headlines questioning if He’s experiments prove otherwise, the scientific...biosecurity, and the entire 2018 saga of “what’s happening in genetics this time?” She lives with her partner... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post...to chat. This can be done randomly or manually assigning which participants to group together. Breakout...doing science, he has assisted many researchers in gaining the confidence to launch new and diverse careers... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...relationships Here are some ways to go about strengthening a new relationship with authenticity and not...(or better yet, become a volunteer). Naturally running into or working with the same people is often the... -
How to Lead a Great Meeting
TypeBlog Post...hour to achieve the purpose of the meeting (see “defining the scope” above) or if you can cut it down to...leader for some tips. Before long, you will be running collaborative, informative, energizing meetings... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post...subject to mishandling? Is the experiment you’re planning to run with the antibodies time-sensitive, using...which you may need in your experiment. If you are planning to conjugate your antibody in the lab, you’ll ... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...with powerful cognitive biases such as motivated reasoning and hindsight bias, we may fool ourselves and ...discoveries Finally, there are good reasons for obtaining a different result after conducting a replication...