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TypeBlog Post...off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target sites from the 18,385...
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What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...lines in B-D represent the pure DNA spectra for comparison. Each of these contaminants will have a slightly... -
AAVs in Retinal Gene Therapy
TypeBlog Post...gene therapy revolution and the development of personalized medicine. References and further reading 1.... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population has, on average... -
Plasmids 101: Colony PCR
TypeBlog Post...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...43:729-736. PubMed PMID: 3907859. 4. Fields, S., Song, O. A novel genetic system to detect protein-protein... -
Plasmids 101: Protein tags
TypeBlog Post... Additional resources: Young CL, Britton ZT, Robinson AS, Biotechnology Journal 2012 , (7): 620-634.... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...boring. I think that such tendency is one of the reasons why we have only three biology-related Nobel prizes... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post... among the earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study ... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post... Rachel Leeson... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...Central PMCID: PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ... -
Bioinformatics at Addgene
TypeBlog Post... Jason Niehaus... -
QC Sequencing Technologies at Addgene
TypeBlog Post.../10.1021/ac2010857 Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post... Zhu, Y., Guan, Y., Wang, L., Liu, M., Feng, B., Song, G., … Li, D. (2024). Adenine transversion editors... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post...2020. His research focuses on the efficiency of transposon-encoded CRISPR/Cas systems in bacteria, specifically... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...plasmid. Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of ... -
An Inside Look into Addgene's Dev Team
TypeBlog Post... area, and when something urgent comes up, that person is the first in line to resolve it for expediency... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...compatible functional livers.” Why are you the best person to do this research? Do you have innovative tools... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...computed during generation of the ICE Score, the Pearson correlation coefficient (r) is also computed and...