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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,045 results for: ins;

Showing: 351 - 360 of 1045 results

  1. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...student and then instructor at Singularity University at NASA Ames, and an instructor at Biocurious.org...makerspaces around the world. Hackerspaces.org maintains a list of active spaces near you.   Learning ...complexity using existing C. elegans mutants. For instance, we use a mutant known as CX51 that exhibits motor...Minnesota has declared our makerspace an educational institution meaning that we can obtain known mutants at $7.00...motor behaviour, and ultimately submit our new strains back to the CGC for other researchers to use. Although...equipment that would otherwise be discarded. For instance, the centrifuge in the picture had a broken controller... the fantastic optics and fluidics platform it contains. Biotech equipment becomes obsolete amazingly ...

  2. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...add a bit of fun. He mixed together 10 E. coli strains producing different pigments, and tasked the students...perfect fit for the streak plate challenge. Chromoproteins are a subset of the fluorescent protein family...off color in ambient light. Expressing these chromoproteins in E. coli would make it easy to distinguish...single colonies, these chromoprotein-expressing strains are easily amenable to other experiments to teach...have the students transform them into different strains of E. coli,” says Westenberg. During the transformation... how different genetic backgrounds of the host strains affect transformation efficiency.  Aside from the...blog Read our other blog posts on fluorescent proteins Check out educational resources on the blog Resources...

  3. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...coding genes in the human genome with fluorescent proteins. For each gene, we needed to design guide RNAs...ranked by specificity of targeting or by cut-to-insert distance for HDR. (The notable exception is TagIn...compressed CFD score) * (gaussian normalized cut-to-insert distance) This gives each guide a score ranging... can be used in the rank equation above. Cut-to-insert distance was fitted to experimental data of its...the greater this distance between the cut and the insert, the more poorly the guide performs HDR. Optimal...sequences to prevent re-cutting of the sequence inserted by HDR. Although this practice is generally advised...enough to prevent re-binding. Lastly, the sequence inserted by HDR may inadvertently re-create a match for...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...modulation of endogenously and exogenously expressed proteins. Here we will review what advantages degron tags... the tagged protein. Degrons are destabilizing domains which will be identified by various enzymes and...manipulate endogenously and exogenously expressed proteins. Thirdly, degrons and their drugs are titratable...not amenable to being tagged, e.g. tagging some proteins can affect their function, the SMASh tag self-...developed as an affinity tag for visualization of proteins of interest. A fluorescent Halo ligand can be ... molecules which target E3 ligases to specific proteins by simultaneously binding both their target protein...their effectiveness with over a dozen different proteins. In the end, you can’t predict with absolute certainty...

  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression...vectors simultaneously express two or more separate proteins from the same mRNA. We've discussed promoters ... detect your gene of interest, scientists have instead developed novel methods to co-express your gene...IRES Elements Translation in eukaryotes usually begins at the 5’ cap so that only a single translation...site, thereby resulting in co-expression of two proteins from a single mRNA. IRES was originally discovered...between your genes of interest and you can then insert the whole multicistronic cassette into a backbone...Although it is recommended to use the 2A peptides instead of an IRES when stoichiometrically equivalent levels...

  6. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...translation of RNA to make proteins. That's central to everything that goes on inside a cell. Until we get ...of enough sensor domains. Ultimately, we'll need a couple thousand or so sensor domains for everything ...DNA systems capable of storing data and computing inside living cells. He's also a co-founder of the BioBricks...BioBricks The BioBrick Foundation's Linda Kahl explains the Addgene collaboration this way: "Addgene is...strategy that organizes how we start the work, and it begins with the central dogma - replication of DNA, transcription...and expensive. There's the promise that we could instead have things like peptide or RNA aptamers that act...

  7. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...for Science.  Cold and overcast with intermittent rains, it was weather befitting of the ominous political...Science and Engineering at Rensselaer Polytechnic Institute. He also studies energy markets in his spare time...cause breast cancer and infertility or to argue against unnecessary clinic regulations, evidence-based ...issues hit hardest. According to the Guttmacher Institute, most women of reproductive age live in a state... how planets form around young stars and the instruments we use to study them. You can follow her at alexandrazgreenbaum.wordpress.com.... I worried that, because we were speaking out against legislators and because the march had been so well-publicized...scientists – including global warming skeptics – insisted that more data and research funding are critical...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    ...Harrach, 2014). Given that the early genes encode proteins essential to initiating the whole process – such...used to house a recombinant sequence (transgene) instead.    Although the late genes are usually present...absent, and the late genes (and their structural proteins, which would encapsidate new virions) are not ... is transient due to the fact that AdV surface proteins (especially fiber’s knob domain, and hexon), as... as well as other intracellularly-exposed proteins and downstream cellular events, all recruit strong ...sense of what AdVs are about – and we hope that it inspires further use of existing vectors, as well as research...resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression...

  9. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...now available. In this post, we’ll review the inspiration behind ExM, how it works, and some of the limitations...most SRM techniques also require specialized instruments and technical expertise, limiting their accessibility...a straightforward answer to the space problem. Instead of imaging smaller, what if we just made the sample...chemical dyes, and genetically encoded fluorescent proteins (Gambarotto, et al. 2019; Tillberg, et al. 2016...adapted for detecting nucleic acids as well as proteins (Klimas, et al. 2023; Wassie, et al. 2019). Perhaps...et al (2021) Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including...Saad O, Bewersdorf J (2020) Light microscopy of proteins in their ultrastructural context. Nat Commun 11...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...classified as a type V CRISPR system. Like Cas9, Cpf1 contains a RuvC-like endonuclease domain, but it lacks ...off-target editing efficiency. Kim et al. and Kleinstiver et al. characterized genome-wide editing efficiency... to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches...essential function in Cpf1-mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with ... are indicated below each graph. Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in ...activity than that previously observed with SpCas9. Kleinstiver et al. suggest that AsCpf1’s off-target rate ...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...
Showing: 351 - 360 of 1045 results