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We narrowed to 1,141 results for: Gene

Showing: 701 - 720 of 1141 results

  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using CRISPR...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Learn about Photoactivatable...proteins. To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins. Solving the...yield, and when transiently expressed in cells, generates much less signal than comparable fluorescent proteins... approach is especially suited to complement optogenetic tools, where light used for excitation can cause...cause unintended activation of the optogenetic system. Biosensors based on Nano-lantern technology are...

  2. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...that control cell cycle gene expression.  Additional Resources on the Addgene Blog Visit the fluorescent...work in Lin Tian’s lab at UC Davis is to generate a genetically encoded fluorescent sensor for serotonin...sequence an individual’s genome. But when they find genetic variants, what does that mean? Previous research...of Washington sought to address the effects of genetic variants in a high throughput manner. She helped...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...GFP Find microbiology blog posts Resources on Addgene.org Find fluorescent protein biosensors Browse Plasmids...

  3. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...PP7, or boxB sequences inserted into the sgRNA generates blue, green, or red colors for labeling. A combination... PP7, or boxB sequences in the same sgRNA will generate additional colors. Image from Ma et al., 2016 ...CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR...PubMed PMID: 30377374. Additional resources on the Addgene blog Download our CRISPR eBook Learn about fluorescent...tools Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR plasmids for visualization...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...as a control. For easier portability, all viral genes are flanked by EcoRI and BamHI restriction sites...coronavirus or COVID-19 research, please email Addgene at help@addgene.org with your order number and so it can .... Sign up for email alerts on the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one...reasons, the QCRG is delighted to be partnering with Addgene, who will be taking over the distribution to expand... coronavirus related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt...

  5. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...Technique Q1: Is there any way to deliver a long gene (>4.5 kB) by AAV? A: One potential strategy is to...applications in diseases which may be linked to epigenetic changes, such as multiple sclerosis?  A: Basic...an exciting area for future AAV application to epigenetic editing. This area is discussed in our recent...excellent questions.    Additional Resources on the Addgene Blog Using virus in your research - a primer for...Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

  6. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...inhibiting gene expression, detecting neuronal signaling, or manipulating any other genetically controlled...wide array of genetic tools for neural interrogation like optogenetic tools, chemogenetic tools, and biosensors... the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral...injected into the brain and efficiently deliver genetic information or tools, making them useful tracers...efficiently infects neurons, directs high-level transgene expression, and has retrograde functionality to...manipulated (e.g., activated or inhibited) by any genetic tool delivered by the AAV.   AAVrg requires high...evaluating how this serotype works in your system. Chemogenetic vectors  Expression of retrograde serotype vectors...

  7. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...telephone, until the message reaches the nucleus where gene expression changes lead to cell proliferation and... on Addgene.org Find nanobody expression plasmids here Learn more about optogenetics in Addgene's optogenetics...induced binding. The lab used this general design scheme to generate two more OptoNBs, one against mCherry...loop 1 always generated OptoNBs with dark-induced binding, but insertion into loop 6 generated both blue ...on the key components of OptoNBs, how they are generated, and their use for protein purification and controlling...nanobody’s ability to bind its target protein.  Generating OptoNBs The opening and closing of the AsLOV2...spatiotemporal resolution than pharmacological or genetic manipulation of the signaling pathway.  As a proof-of-principle...

  8. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ... education policy. Additional Resources on the Addgene Blog Synthetic Biology and the Importance of Assembly...All Synthetic Biology Blog Posts Resources on Addgene.org Guide to SynBio Assembly Standards Find Plasmids...

  9. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...at East Tennessee State University. He works with gene expression in Pseudomonas aeruginosa. Follow him...Protocols Check Out How Addgene Develops Lab Management Software Resources on Addgene.org Find Protoctols ... and do not necessarily represent the views of Addgene. I think it’s safe to assume that anyone reading...by New England BioLabs In my lab we do a lot of genetic construction and cloning experiments. Not all of...100percentSDS.     Additional Resources on the Addgene Blog Learn How to Keep a Lab Notebook for Bioinformatic...

  10. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification systems...Results Use REPLACR Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Check Out Our Plasmid...newly synthesized product from a site-directed mutagenesis experiment? The answer to both questions is the...cleave DNA. DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired...mutation into your plasmid of interest by PCR generates mutated plasmids with no methylation (there are...illustrates how sequence visualization software (we used Snapgene here) can help you assess whether your restriction...describing dam-/dcm- E. coli Resources on the Addgene Blog Keep up with the Latest Cloning Techniques...

  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition to using...review how CRISPR-derived base editing works in general. Base editors use either catalytically dead Cas9...efficiency and produced fewer indels than first-generation editors. This team also demonstrated that different...sequences) (Huang et al., 2024). BE4max, a CBE, generated similar deletions at a lower frequency. Their ...and its inclusion significantly reduced indels generated by CGBEs. What's next? Base transversion editing...base transition editing, we can expect several generations of optimization before the field begins to converge...

  12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...In this case, Konermann et al. targeted MAPT, the gene encoding dementia-associated tau, delivering dCasRx...possibilities. To identify Cas13d, both groups used very general bioinformatic screens that looked for a CRISPR ...scientific sharing will continue to grow, and we at Addgene appreciate our depositors for making their tools...29551272 Plasmids from this paper are available at Addgene. Yan, Winston X., et al. “Cas13d Is a Compact ...29551514 Plasmids from this paper are available at Addgene. Learn more about research at Arbor Biotechnologies...Biotechnologies. Additional Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips...Cas13a (C2c2) Characterization Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR...

  13. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...situ is the knock-in of fluorescent tags into the gene locus of interest. An advantage of this alternative...studied a synaptic protein that is linked to neurodegeneration. The level of this protein is decreased in...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...protein-protein interactions Deposit plasmids with Addgene! ...

  14. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... widely available and applied by researchers for gene targeting. Fast forward a decade, and the CRISPR...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook... trans activity. AsCas12a, on the other hand, generates staggered DNA nicks at its target in cis and cleaves...end goal. CasPEDIA also features useful links to Addgene resources throughout the site. In the Experimental...each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that enzyme... from the article if they're available through Addgene. So whether you're looking for the exact Cas9 plasmid... or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered!     ...

  15. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...where we could incorporate cold-tolerance inducing genes within a regular mesophilic host system. This would...resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent...

  16. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...repair of Cas9-generated breaks is useful if the intent is to make a knockout in your gene of interest, ..., as it is prone to generating indel errors. Indel errors generated in the course of repair by NHEJ are...are typically small (1-10 bp) but extremely heterogeneous. There is consequently about a two-thirds chance...Of some importance, the deletion can be less heterogeneous when constrained by sequence identities in flanking...overhangs is available. Cas9 can also be altered to generate a targeted single strand break; when two such ...  Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology, the Dept. of Biochemistry... Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen...

  17. RNA Extraction Without A Kit

    Type
    Blog Post
    ...downstream application such as quantitative PCR to assess gene expression. There are several things that researchers...everything, including your skin. Here are some general tips for working with RNA, even if you are using...10.1038/nprot.2006.83 Additional resources on the Addgene blog Read blog posts related to plasmid protocols...for purifying DNA without a kit Resources on Addgene.org Check out our molecular biology protocols Find...

  18. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...You realize, much too late, that you labeled your gene of interest incorrectly on slides 8-16. Your notes...remember related to this paper and/or biology in general until I reached Fig. 6, Panel L, and hope nobody...breath–you got this!  Additional resources on the Addgene blog Transferable Skills Guide: Time Management...

  19. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... useful as a mechanism of transient knockdown of gene expression. This system could be used in lieu of...complementary site in the genome where it will proceed to generate a blunt DNA double strand break. Cas9 has been...Cas12’s primary difference from Cas9 is that it generates staggered breaks, also known as sticky ends. Cas12a...targeting experiments requiring single strand DNA generation, such as homology directed-repair. Cas12a is ... of DNA. This makes it particularly useful in generating transient edits of signaling molecules (RNA) ...If your genome engineering goals expand beyond generating frameshift mutations or single base pair edits...s41586-021-03886-5 Additional resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for ...

  20. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...Chemical Modifications of CRISPR RNAs to improve Gene-Editing Activity and Specificity. JACS 144, 12584...resources on addgene.org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources...effectively serving their function of guiding Cas9 to generate a targeted break. These advances in gRNA technology...or immunity itself is being studied. Check out Addgene’s validated gRNA repository to find pre-tested gRNA...photocleavable gRNAs. Photocleavable guides are generated by introduction of a single photocleavable 2-nitrobenzyl...lentiviral plasmids Additional resources on the Addgene Blog  How to design your CRISPR gRNA CRISPR 101...
Showing: 701 - 720 of 1141 results