We narrowed to 775 results for: Mos

...termed PITCh (Precise Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell...

...protein such as GFP? All of these outcomes can be most effectively achieved with different CRISPR components...

...Institute) has consistently been one of the repository’s most frequently requested plasmids. I wondered how many...

...), decided to take a different approach. His own most impactful learning experiences happened in the lab...

...available to evaluate AAV vector performance. Yet, most of the technical data needed to replicate and build...

...cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker for IF. However, you ...

...42% editing efficiency. They note that RESCUE is most active in a 30 nt guide when C or U base-flips are...

...virus in reaction mix D = Dilution factor of virus Most droplet readers have a few channels for detecting...

...work in the future; they are transferrable across most, if not all, industries and work environments. This...

...because CpG dinucleotides are underrepresented in most vertebrate genomes (Josse et al., 1961, Swartz et...

...numerous genomic loci through proteomics and the chromosome conformation capture (3C) technique. In addition...

... region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate to begin working...

...common lab cloning strain has failed so far. What is most important is the transformation efficiency of the...

...overexpression library identified several overlapping genes, most genes were unique to the individual libraries. These...

...   Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study...

... other edits Like I said earlier, HDR is not the most common editing pathway for DNA repair. To tilt the...

... R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases...

...Designing colony PCR primers The first and perhaps most important step to colony PCR is designing primers...

...will increase the stringency of your binding. And most importantly: Independently validate identified binding...

...does not mediate detectable off-target cleavage. Most gRNAs directed low-frequency Cpf1 cleavage at 1-...