We narrowed to 765 results for: Mos

1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ...PRIDICT saves time and resources by selecting the most promising pegRNA designs and especially facilitates...

2. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

   ...modified strains that contain your newly tagged gene. Most insertions can only be detected by labor-intensive...

3. Magnetic Control of Proteins: More than a Dream

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   ...humans (like many organisms) are opaque, which makes most biomedical applications of these tools difficult...

4. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   ...NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years...

5. Hot Plasmids and Viral Preps - May 2021

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   ...RBD responsible for binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each...

6. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   ...Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis...

7. PITChing MMEJ as an Alternative Route for Gene Editing

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   Blog Post

   ...termed PITCh (Precise Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell...

8. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   ...protein such as GFP? All of these outcomes can be most effectively achieved with different CRISPR components...

9. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ...work in the future; they are transferrable across most, if not all, industries and work environments. This...

10. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...   Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study...

11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ... R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases...

12. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...42% editing efficiency. They note that RESCUE is most active in a 30 nt guide when C or U base-flips are...

13. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ...), decided to take a different approach. His own most impactful learning experiences happened in the lab...

14. CRISPR 101: Making a Knock-In Cell Line

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    ... other edits Like I said earlier, HDR is not the most common editing pathway for DNA repair. To tilt the...

15. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ...numerous genomic loci through proteomics and the chromosome conformation capture (3C) technique. In addition...

16. Tips for arabidopsis transformation

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    Blog Post

    ... region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate to begin working...

17. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...common lab cloning strain has failed so far. What is most important is the transformation efficiency of the...

18. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

    ...overexpression library identified several overlapping genes, most genes were unique to the individual libraries. These...

19. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...available to evaluate AAV vector performance. Yet, most of the technical data needed to replicate and build...

20. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ...cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker for IF. However, you ...