We narrowed to 772 results for: Mos

1. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...the city of Guangzhou and see some of the sites. Most fascinating to me was the outdoor market where one...

2. The Materials Science of Optogenetics Experiments

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   Blog Post

   ... does require a significant amount of equipment, most of which is relatively cheap). Several protocols...

3. Hot Plasmids: Summer 2025

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   ...Get your sunglasses — these proteins are bright! Most current yellow fluorescent proteins tend to photobleach...

4. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...modified strains that contain your newly tagged gene. Most insertions can only be detected by labor-intensive...

5. Magnetic Control of Proteins: More than a Dream

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   ...humans (like many organisms) are opaque, which makes most biomedical applications of these tools difficult...

6. Viral Vectors 101: Producing Your rAAV

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   ...simplex virus to facilitate efficient replication. The most common production system, however, is a “helper-...

7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   ...PRIDICT saves time and resources by selecting the most promising pegRNA designs and especially facilitates...

8. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   ...NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years...

9. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   ...Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis...

10. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ...RBD responsible for binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each...

11. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    ...termed PITCh (Precise Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell...

12. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post

    ...protein such as GFP? All of these outcomes can be most effectively achieved with different CRISPR components...

13. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...Institute) has consistently been one of the repository’s most frequently requested plasmids. I wondered how many...

14. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...   Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study...

15. MXS Chaining

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    ...because CpG dinucleotides are underrepresented in most vertebrate genomes (Josse et al., 1961, Swartz et...

16. Tips for arabidopsis transformation

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    ... region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate to begin working...

17. Simplify Cloning with in vivo Assembly

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    ...common lab cloning strain has failed so far. What is most important is the transformation efficiency of the...

18. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...overexpression library identified several overlapping genes, most genes were unique to the individual libraries. These...

19. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ...numerous genomic loci through proteomics and the chromosome conformation capture (3C) technique. In addition...

20. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ... R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases...