We narrowed to 741 results for: REV

1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   ...deficient in dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes...

2. Plasmids 101: Biotinylation

   Type

   Blog Post

   ...in different microenvironments. There are also reversible and cleavable biotinylation reagents to help ...

3. 10 Steps to a Perfect Science Talk

   Type

   Blog Post

   ...given a 50 minute talk (not a lab meeting) in the previous year. Usually fewer than 10% have done so. How...

4. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   ...screening for succesfully mutated clones. Forward and reverse primers are designed to be complementary, but each...

5. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   ...Sequence and Ligation Independent Cloning (SLIC) in a previous Plasmids 101 post. Although SLIC may be more cost...

6. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

   Type

   Protocol

   ...precautions, tuck your sleeves into your gloves to prevent potential spills down your sleeve. This may or ...

7. Protocol - How to Streak a Plate

   Type

   Protocol

   ...each new section crosses at least one line of the previous section so that it will contain some bacteria....

8. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   ...https://doi.org/10.5607/en20006 Rolland, A.-S., Kareva, T., Yarygina, O., Kholodilov, N., & Burke, R. ...

9. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   ...publications that cited the antibody, consider reviewing the materials and methods sections of those publications...

10. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    ...Wenning Qin and Haoyi Wang. CRISPR/Cas9 is revolutionizing the mouse gene-targeting field. Mice have long...

11. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    ...disrupt the target site for the nuclease thereby preventing re-cutting. Transposase removal of the cassette...

12. Finding Your Perfect Job After University

    Type

    Blog Post

    ...work-life balance compared to working in research. My previous experience working in Industry was really valuable...

13. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...responses to the mid and post-program self-assessment revealed that their knowledge and skills had improved significantly...

14. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type

    Protocol

    ... MfeI - CAATTG ). The bottom oligo will be the reverse compliment so that they can anneal. Top oligo: ...

15. Centrifugation

    Type

    Protocol

    ...given as Relative Centrifugal Force (RCF) or Revolutions Per Minute (RPM), these are not the same units...

16. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    ... strategies in C. elegans As brilliantly and irreverently illustrated in the parable of the Geneticist...

17. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    ..., and time to the cause ever since. Unlike the previous two team types, the skills are not cherry-picked...

18. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    ...Additional Resources on the Addgene Blog Read Our Previow Post on the March for Science Learn How Future ...

19. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type

    Protocol

    ... vector, you will need to use a phosphatase to prevent re-circularization of the vector if you are cloning...

20. Immunocytochemistry

    Type

    Protocol

    ...antibody concentration will vary between antibodies. Review the manufacturer's instructions before starting...