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We narrowed to 954 results for: Tes

Showing: 701 - 720 of 954 results
  1. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... including the recent homepage and search tool updates. Now, let’s take a look at the least visible part...behind Addgene’s new search  Like millions of other websites, we built our new search engine atop Elasticsearch...
  2. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...instead of room temperature or 37 °C. The protocol notes when there are different incubation options available...empirically determined. If possible, run an initial test with serially diluted primary antibody and determine...Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel...VWR 76322-150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, ...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize... and incubated overnight with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary...
  3. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...load The Wikipedia definition of cognitive load states: “cognitive load refers to the used amount of working...even the most enthusiastic of learners.  What contributes to cognitive load?  Everything! Here are some...
  4. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...idea of the basics of immunoimaging. A scientist conjugates an antibody with a signaling molecule, the antibody...secondary antibodies on hand and use them to easily test different antibodies or different signaling molecules...
  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...weeks to increase the antibody titer. The titer is tested following each immunization until the desired level...
  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...theaters, the Razr was the hottest phone on the market, and two new websites, Facebook and Gmail, were ...
  7. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...and isolated with antibiotic selection. The team tested this system in vitro and in vivo. They identified...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...
  8. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...(Japanese, German, and English) and even on 60 Minutes. We are blessed with a large community of scientists... employee pool and we get a lot of successful candidates from internal referrals. Our long reaching reputation...
  9. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough for this approach... guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik....
  10. The time and cost required to make a plasmid

    Type
    Blog Post
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...
  11. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...below): 1. Resistant cells among the separate replicates would contain the same labels - i.e. they would...subpopulation 2. Resistant cells among the separate replicates would have different labels - i.e. they would...
  12. A Look at Addgene's QC Process

    Type
    Blog Post
    ...the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature...at a best practices meeting, where the QC team updates and reviews our sequencing QC standards to ensure...
  13. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other plasmids...undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be small and poorly ...
  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...transfer plasmid, the component that physically integrates into the host genome, the virus is replication...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate in the waste...
Showing: 701 - 720 of 954 results