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...suggestions for using LinkedIn and Facebook. For each of the three social media platforms, there’s some...blog writing tips are elements I try to include in each post I write for the Addgene blog. 5. "Social Media...

...slides, learn from audiences as they do science outreach and engage with the public, and learn from the...science communication to more graduate students.   Each June, graduate student leaders in science communication...

...require a protein to be tagged, which limits these approaches to use with recombinant proteins. Purifying an...lot like a game of telephone, until the message reaches the nucleus where gene expression changes lead ...

...conventional breeding; synthetic biology is required to achieve this novel and unique phenotype. It’s an unexpected...flowers, bugs, and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from ...

...and effectively presenting the value tailored to each and every audience uniquely (not a focus in graduate... usually) is one of the key skills required to achieve success in this business. A good presentation can...

...important to establish the type of audience you want to reach with your paper. Is it the wider scientific community... and the entire referee report are permanently attached to the published article. In addition, authors...

...if desired, but it's not required for their use. Each RANbody is detectable by two different tags: a reporter...particular applications. Read on to learn more about each reporter or check out table 1 for a summary. HRP...

...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...

...There are lots of ways scientists communicate with each other: from formal mechanisms like publishing a ...potential collaborators, blogging and more. These each have different goals, formats, and processes, and...

...specificity. They found that eSpCas9(1.1) and SpCas9-HF1 each stall in an inactive form after binding to a target... Will combining the different mutations found in each of these great tools further enhance genome editing...

...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...

...efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible Cas enzymes Are we truly PAM-less? Have we reached an essentially PAM-free solution? Almost! Assuming...

...expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than in the...expression and downstream monitoring. This targeted approach allows scientists to focus on specific cell populations...

...interactions. One popular approach is to fuse a protein of interest to each part of a split fluorescent...

...infect more HEK293 cells and produce more rAdV to reach higher titers. Successfully infected cells will ...at increasing scale over the course of 1-2 weeks. Each round of amplification should result in a 10-100...

...teams of three in sections of 15 students. Within each section, teams are assigned different groups of ...research question, the plasmids are also used to teach students about restriction mapping and western blotting...

...yield and higher absorption than it’s predecessor, sREACH, allowing for reduced artifacts due to residual...greater extinction coefficient when compared to sREACH. Find the FLIM-FRET acceptors here. See Addgene's...

...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...about the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update ...

...expression Generally speaking, one may want to achieve either inhibition or activation of neurons in a...Stoica et al., 2013). Vector delivery may also be achieved via injection into the liquor of the cerebral ...

...share a common genetic background, which means that each mouse is genetically identical and all mice of the...genetically identical mice. Thus, scientists must genotype each animal to identify the ones with the desired genotype...