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We narrowed to 949 results for: ache

Showing: 701 - 720 of 949 results

  2. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...share a common genetic background, which means that each mouse is genetically identical and all mice of the...genetically identical mice. Thus, scientists must genotype each animal to identify the ones with the desired genotype...

  4. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than in the...expression and downstream monitoring. This targeted approach allows scientists to focus on specific cell populations...

  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...

  6. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible Cas enzymes Are we truly PAM-less? Have we reached an essentially PAM-free solution? Almost! Assuming...

  7. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ... First, the individual sequencing reads (~150 bp each) are aligned to the reference sequence of the plasmid...are most likely to correspond to the contaminant. Each contig is then BLASTed against the NCBI nucleotide...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...relatively high efficiency. These alterations are achieved through DNA degradation, thanks to the unique .... Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better option. To...

  10. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...fluorescence intensity decreased whenever the magnet approached the sample (by less than 1%) and recovered after...MagLOV will be possible with other engineering approaches and that magnetosensitive tools can be developed...

  11. Of Myc and Men

    Type
    Blog Post
    ... that this step “...proved to be quite easy to achieve…” - but I think we can all agree that it sounds...generate a reliable antibody for protein tagging approaches” was not part of the goal when Evan’s team generated...

  12. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...most anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated spike protein and a ...specific anti-SARS-CoV-2 antibodies (Fig 1B). This approach can augment viral surveillance by enabling rapid...

  13. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...avaliable for less than than it would cost to buy each plasmid in the kit individually. If I order the...more than one gene inserted into a backbone, and each is given its own gene/insert section on the plasmid...

  15. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...fluorescent protein Excitation & Emission (ex/em): Each FP has its unique ex/em peak. Therefore, choose ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...

  16. Western Blot

    Type
    Protocol
    ... can be adapted for wet and semi-dry transfer approaches. Last Update: January 24, 2022 Workflow Timeline...to load an equivalent amount of total protein for each sample. Pro-Tip The ideal total protein loaded will...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... great promise in preclinical studies and could reach clinical trials soon. The AAV safety profile is...choroideremia, X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing...

  20. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...genes and which applications are best (or worst) for each antibiotic. The use of antibiotic resistance DNA...a look for a quick recap of the pros and cons of each antibiotic and happy selecting!   Antibiotic...
Showing: 701 - 720 of 949 results