We narrowed to 958 results for: ache

...important to establish the type of audience you want to reach with your paper. Is it the wider scientific community... and the entire referee report are permanently attached to the published article. In addition, authors...

...if desired, but it's not required for their use. Each RANbody is detectable by two different tags: a reporter...particular applications. Read on to learn more about each reporter or check out table 1 for a summary. HRP...

...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...

...There are lots of ways scientists communicate with each other: from formal mechanisms like publishing a ...potential collaborators, blogging and more. These each have different goals, formats, and processes, and...

...specificity. They found that eSpCas9(1.1) and SpCas9-HF1 each stall in an inactive form after binding to a target... Will combining the different mutations found in each of these great tools further enhance genome editing...

...infect more HEK293 cells and produce more rAdV to reach higher titers. Successfully infected cells will ...at increasing scale over the course of 1-2 weeks. Each round of amplification should result in a 10-100...

...teams of three in sections of 15 students. Within each section, teams are assigned different groups of ...research question, the plasmids are also used to teach students about restriction mapping and western blotting...

...yield and higher absorption than it’s predecessor, sREACH, allowing for reduced artifacts due to residual...greater extinction coefficient when compared to sREACH. Find the FLIM-FRET acceptors here. See Addgene's...

...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...about the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update ...

...expression Generally speaking, one may want to achieve either inhibition or activation of neurons in a...Stoica et al., 2013). Vector delivery may also be achieved via injection into the liquor of the cerebral ...

...share a common genetic background, which means that each mouse is genetically identical and all mice of the...genetically identical mice. Thus, scientists must genotype each animal to identify the ones with the desired genotype...

...interactions. One popular approach is to fuse a protein of interest to each part of a split fluorescent...

...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...

...efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible Cas enzymes Are we truly PAM-less? Have we reached an essentially PAM-free solution? Almost! Assuming...

...expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than in the...expression and downstream monitoring. This targeted approach allows scientists to focus on specific cell populations...

... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ...

... First, the individual sequencing reads (~150 bp each) are aligned to the reference sequence of the plasmid...are most likely to correspond to the contaminant. Each contig is then BLASTed against the NCBI nucleotide...

...fluorescence intensity decreased whenever the magnet approached the sample (by less than 1%) and recovered after...MagLOV will be possible with other engineering approaches and that magnetosensitive tools can be developed...

...relatively high efficiency. These alterations are achieved through DNA degradation, thanks to the unique .... Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better option. To...

... that this step “...proved to be quite easy to achieve…” - but I think we can all agree that it sounds...generate a reliable antibody for protein tagging approaches” was not part of the goal when Evan’s team generated...