We narrowed to 716 results for: cas

1. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   ...that means saying no to a negotiation or, in this case, to academia altogether.   The primary point of ...

2. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   ...undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation...

3. Technique: Probe Phage Genomes for Host Binding Proteins

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   Blog Post

   ...what her cat is thinking. Find her on Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al...

4. When Fidelity Matters: A frank discussion about ligase fidelity

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   Blog Post

   ...lacking gaps or mismatched base pairs (as in the case for LCR, LDR and Padlock Probes), use of a high ...

5. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   Blog Post

   ...Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol...

6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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   Blog Post

   ...of AAV vector we produce containing a DIO/FLEx cassette using next-generation sequencing (NGS) (Guerin...

7. A Guide to Getting Started in Undergrad Research

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   Blog Post

   ...think reflecting on why it is we enjoy, or in some cases, don’t enjoy, is worthwhile and can help us make...

8. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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   Blog Post

   ...other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better tools...

9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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   Blog Post

   ...and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast...

10. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post

    ... (VEGF) in tumors is attributed to a signaling cascade, where lack of oxygen in the tumor, activates hypoxia...

11. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ...acceptor fluorophores, it’s best to consider these cases separately when talking about the tools that are...

12. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    ...titer must be determined empirically (this is the case for any viral injection into the brain). As a general...

13. Cloning

    Type

    Guide

    ...promoters and into backbones with different selection cassettes). Although gateway cloning is a simple and efficient...

14. Selecting Your Plasmid Purification Kit

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    Blog Post

    ...step and provide troubleshooting advice. In these cases, it can be helpful to select a kit with easy-to-...

15. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    ...range of possibilities in clinical situations. In cases where the objective is to fix a disease-inducing...

16. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...concentration to detect low levels of proteins. In this case, it might be faster to run the same amount of protein...