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... directed to the proper target sequence. They succeeded in labeling two different pairs of chromosomes...

...variety of useful resources and protocols! We invite feedback from all members of the research community to ...

...duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice. This can result...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

...’re just starting out with prime editing, you’ll need to design and optimize a pegRNA for your particular...

...direct transfer from one cell to another). Bacteria need special fertility genes to be capable of conjugation...

...Arg) amino acids more frequently than any other. Indeed, nearly 20% of the single missense mutant alleles...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

...Another potential limitation of Split-BioID is its speed of labeling. BioID requires 6-24 hours for tetracycline...

... the insertion of the GFP tag precisely where we need it, in this example, at the C-terminus of the gene...

... a Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. ...

... single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded optical space for multiplexing...

...sample. The PCR is then performed, optimized as needed, and cleaned up using a standard PCR cleanup kit...

...deliver gene therapies directly to the cells that need them. Adeno-associated virus (AAV) – based delivery...

...volume. Always remove packaging cell debris before proceeding to ultracentrifugation to avoid contaminating...

...Plasmids 101 eBook to get all the background you need to start working with plasmids! What are termination...

...fluorescent markers are used as it eliminates the need for antibody staining. Check Out Our In-Depth Fluoresence...

...datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As far as...

...microphone system ready for each panelist if the room needs it (lapel mics are best). The moderator takes control...

...yellow fluorescent proteins just haven’t met your needs, consider trying mGold2s or mGold2t for your next...