...development happening at Addgene under the hood. A significant portion of our development may not be visible...ourselves. We are also able to consider different technical approaches that may come up during discussion.... information. It is quite possible that the clarifications lead to the story being scrapped or broken ...While writing the code, we may need additional clarification and again we collaborate with the Product Team... been updated in several years) or they make significant changes that make it incompatible with our software...software from Python 2.7 to Python 3.5. There are significant changes to the language and it took us a total...

...Societies, such as the American Society for Cell Biology (ASCB) or the American Chemical Society (ACS) are ...often scarce and difficult to identify. So what specifically should you do? What follows are just a few suggestions... in fact, some of the very things that were so critical to me landing my own position! Conferences One...know all in one place! This information can be critical to a focused and effective job search. As with...

...possess cell walls. As a result, Mycoplasma are intrinsically resistant to this class of antibiotic. Acquired...Microbes (bacteria included) are very skilled at the delicate art of survival. Existing virtually ubiquitously...of intentions. The take-home? Always take your medication as prescribed. How do we know which bacteria ...When antibiotic resistance testing is requested clinically, this involves growing a standardized amount ...-trusted method that is frequently used in the clinical laboratory. What can we do to stop the emergence... is passionate about health equity, science communication, and public health.     References 1. O'Neill...

...regions and allocate specific functions to them. Electrical recordings and lesioning studies have been essential...within the LC of mice. When those neurons are chemogenetically activated, norepinephrine release causes a...test has been used since the 1970s to test the efficacy of antidepressant treatment in rodents. In this...responsible for the antidepressant effect. To specifically activate the Drd1 neurons, they injected AAV2... "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress."...long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390. PubMed...

...FRET during a journal club, my guitarist brain automatically thought about the raised element found on the...allows the detection of molecules' interactions, modifications or dissociations in situ. Used since the mid...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...Periasamy defined FRET as "a distance-dependent physical process by which energy is transferred non-radiatively...highest absorbing acceptor and fluorophores with significant overlap in their spectra. The pair CFP-YFP was...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells... promoters only work in prokaryotic cells and typically only in the same or closely related species from... be ways to turn them on or off at will with a chemical, heat, or light. In the cell, promoters themselves...scientists have created synthetic promoters, which typically include some combination of other promoter elements...Inducible with Tetracyline or its derivatives.  Typically contains a minimal promoter with low basal activity...

... the planning section below, it’s important to dedicate specific amounts of time to specific tasks beforehand...activities that are needed to accomplish these goals. Practically speaking, this means I write everything in a ...down? It’s more viscerally appealing for me to physically cross things out (it just feels good) and I remember...instance, if I don’t have draft blog post ready for publication for tomorrow, I’d better upload that post from...handle doing a particularly annoying task and then dedicating that much time to it each day. Once you get over...Student Community Learn about Careers in Science Communication Resources at Addgene.org Check out Careers...

...percentage of survey respondents indicated that cost was a significant barrier to ELN adoption. This includes...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...to digital for example): “… 99% of respondents indicated that ease of use would influence their ELN choice...collaborative functionalities include comments, notifications, sharing files, assigning team members to certain... step of the research process will save them significant amounts of time and effort and give the lab a...

... minus sign (-) as is typically standard; just having the gene listed indicates it is non-functional. ...that carried out the labor-intensive process of replicating your new plasmid: the Escherichia coli bacteria...conjugation. Genes listed on F´ are wild-type unless indicated otherwise fhuA (formerly tonA) ferric hydroxamate...vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96... Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors...

...Vincent and Niroshi Senaratne, from the Biological and Biomedical Sciences program at Harvard University...and Genomics at Harvard Medical School, and is currently a fellow in clinical genetics at the University...the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...

...target proteins of interest for labeling, quantification, purification, chromatin immunoprecipitation and more...created the Monoclonal Antibody Research Institute dedicated to: 1) developing new ways of generating antibodies...repositories provide standardized quality-control and technical support to the community. Thanks to Our Guest ...Freedom: The Expansion of Data Sharing in Research Publications ...

...Niroshi Senaratne and Ben Vincent from the Biological and Biomedical Sciences program at Harvard University...and Genomics at Harvard Medical School, and is currently a fellow in clinical genetics at the University...the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...

...the sequence of interest. 5. Use a silica spin column for purification of the samples you send for DNA sequencing...requires you to perform your own Big Dye PCR amplification reaction (as opposed to using the all inclusive...Acetate/isopropanol precipitation method or using a silica spin column available from several vendors. The...image below), so I would strongly recommend using a silica spin column. They can be pricey, but well worth...

...reason for that. Lately, significant advances in the understanding and application of CRISPR/Cas9 technology... by even splashier news: Jiahao Sha of Nanjing Medical University had successfully used the CRISPR/Cas9...caused by genetic abnormalities, targeted genetic modification in monkeys is invaluable for the generation ... Times feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ... as these recent blog posts on prime editing, IgnaviCas9, and Nanoblades), but we hope that this blog ...engineered tRNA adenosine deaminase (TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called... toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing) mediates targeted...(A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting Cas13 (dCas13) with...

...you’ve saved. Repurposing regulated medical waste Regulated medical waste (RMV), which we collect in those...companies to obtain these labels! Reusing plastic Historically, scientists washed and reused materials such...flow cytometers. These fluidic machines handle biological samples and are cleaned between each use. You...those red biohazard bags, typically is destined for a landfill. However, Triumvirate Environmental developed...

...Scientists series will focus on an aspect of communication especially important for a manager – getting... Creating a culture of excellent, effective communication between all members of a team. As described ...what is going on and can contribute. Members periodically break, go exploring outside the team, and bring... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... opinions in meetings, via email, at formal communications events or at informal gatherings, like at lunch...

...problem is that scientists sometimes forget how to communicate about their work with non-academic people.” Daniel...while - a tool to expand perception, which is ironically what science is about. For me it is essential... time, defend the thesis, etc. Photography is critically important in many fields of biology and I believe...appreciated by a wider audience for more than just its biological importance. More of Daniel's stunning work can... new method to explore the inner structures of delicate plants and other organisms without destroying ...

...coming together, we can create a significant impact on both clinical care and public health for this current...match up scientists across fields with different technical knowledge that could help fight COVID-19. They...funding and volunteer opportunities, protocols, publications, and more to accelerate the pace of research...is based in Canada, much of the information is applicable to researchers worldwide. “COVID-19 has spread...

...imaging method that uses iterative staining and chemical bleaching to allow for high-resolution imaging...not recommended reagents) along with information critical for the performance of a reagent, e.g., antigen... repository for reagents, protocols, panels, publications, software, and datasets. It’s also a great place... (2022). IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse...Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial...