Simplify Cloning with in vivo Assembly
Type
Blog Post
...cloning strain has failed so far. What is most important is the transformation efficiency of the strain...
We narrowed to 760 results for: mpo
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New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post... enough for efficient library performance, an important criterion for probing a limited number of cells... -
Great Results Start with Great Standard Curves
TypeBlog Post... or very similar buffers if not, since buffer components can affect the final readout. When preparing ... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...al. Before picking a secondary nanobody, it’s important to review a few characteristics of your primary... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...excess dye from the sample, which is particularly important for samples where washing is difficult, such as... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...genome. But not all of those 13,000 regions are important for maintaining ESCs. So which of these 13,000... -
CRISPR Antimicrobials
TypeBlog Post...species, or even an antibiotic resistance gene. Importantly, native, non-pathogenic bacteria remain to help... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...increase the stringency of your binding. And most importantly: Independently validate identified binding partners... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...misread. 2. Track the container - It is just as important to track the location of the container housing... -
Plan Your Experiment
TypeGuide...you are ready to start designing the specific components (Cas enzyme and guide RNA) for your experiment...genetic manipulations require different CRISPR components. Selecting the genetic manipulation you plan ...plan to perform will help you narrow down which components you’ll need for a given experiment. In the table... will cover strategies for designing the gRNA component for each category. Genetic Manipulation Application...testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference : Target...Predicted On-Target and Off-Target Activity Another important factor to keep in mind when designing your gRNA...In addition to off-target activity, it is also important to consider factors that maximize cleavage of ... -
Hot Plasmids - August 2020
TypeBlog Post...GRABNE) sensors allow for sensitive and high spatiotemporal measurements of norepinephrine. These norepinephrine... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...ES, Koonin EV, Zhang F (2016) C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector... -
An Inside Look into Addgene's Dev Team
TypeBlog Post.... Third party libraries are reusable software components that can be incorporated into software. You can... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...Plant base editor platforms and their molecular components. Adenine base editors for plant We developed... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post... amplify well and others don’t. The size and composition of your library can drastically impact what conditions... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post... penetration depth as well as high spatial and temporal resolution, making it ideal for visualizing gene... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...complex type III-A and III-B systems. Though it was composed of many protein subunits, it was nonetheless a... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...to be adjusted depending on the sample. It is important to remember that sample lysates will also contain... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post... Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com... -
Bioinformatics at Addgene
TypeBlog Post...this two-part blog series, we will describe the importance of bioinformatics at Addgene and how our search...