We narrowed to 903 results for: nes

1. When Fidelity Matters: A frank discussion about ligase fidelity

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   ...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013...

2. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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   .... Yu, J., et al., Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007....duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced ...

3. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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   ...of prime editing tools. We’ll summarize the major ones in Addgene’s repository later in the post. Advantages...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...

4. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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   ... knowledge to inform on the qualities of what determines good sgRNA design. Incidentally, the first software...Cas-OFFinder will suffice. Cas-OFFinder provides a bare-bones data dump of ALL strings in a genome using any IUPAC...

5. CRISPR 101: Cytosine and Adenine Base Editors

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   ...above 30% for a variety of targets in human cell lines, with an average indel frequency of only 1.1%. These...which is significantly more effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following...

6. Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!

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   ...grade DNA!     This is just the first of many milestones Addgene is poised to reach in the near future...

7. 2022 Depositor Week

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   ...this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame  Thursday...

8. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

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   ...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to Our...

9. Addgene's 20th Anniversary Party

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   ...words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein were also on ...

10. It’s CRISPR Week at Addgene!

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    ...focus on generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll...

11. Lucky Thirteen: Guest Contributors of 2023

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    ...)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie...

12. New FLIS Plasmid (April Fools 2022)

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    ...Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that can be activated...

13. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

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    ...) ourselves back into our regular post-holiday routines, why not take a little time to appreciate the ...

14. Congrats to the 2019 Deck The Lab Winners

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    ...from lab gloves. The tree was adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape...

15. Enabling high school research at the Journal of Emerging Investigators

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    ...published their work on a protein required for cytokinesis in JEI. Developing an interested in biology, ...

16. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

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    ...emotions (while you’re on a break from expressing some genes of interest).   Blugene is so excited they...

17. Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart

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    ...creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...

18. Expanded Access to Rabies Plasmids

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    ...will be removed before your account is charged.  Timelines  We have offered VSV and SARS-COV-1 plasmids under...

19. Bringing Renewable Energy to Addgene

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    ...aggregation program” for all local households and businesses. Electricity aggregation allows a community like...

20. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

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    ...) 26:00-28:39 : Methods for Delivering Multiple Genes to Cells for Gene Therapy 28:40-35:10 : GFP Activatable...