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  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...Institute have supported the AAV initiative from the beginning and have worked closely with us to functionally... isn’t the only facet of a project this big. Maintaining our excellent service and accuracy involves caring...

  3. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...subject to mishandling?  Is the experiment you’re planning to run with the antibodies time-sensitive, using...which you may need in your experiment.  If you are planning to conjugate your antibody in the lab, you’ll ...

  4. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...manipulate what this cargo is and where it goes, turning these pesky germs into useful tools. Here, we will...Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et al. “Altering the tropism of lentiviral vectors...

  5. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your...and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C in APC with the IgG2c subclass...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...you are in the design phase of your experimental planning, you need more nitty-gritty details — how can ...link to search Addgene's collection for plasmids containing that enzyme, and embedded in the References lists...

  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...command line, and so was really starting from the beginning. Luckily, a fellow graduate student and a data... entered is useful for beginners who are still learning the basics. However, these notes take up a lot...

  8. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ... for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). If the reviewers...future. Disregard reviewer’s comments without explaining why. If it looks like you have ignored their ...

  10. dTAG - You're it!

    Type
    Blog Post
    ... additionally provides detailed information on cloning procedures used to construct the deposited plasmids...Considerations when getting the dTAG system up and running in your lab Confirm the functionality of your fusion...

  11. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...target opposite DNA strands and specify a large intervening deletion. Not only can this method precisely ...induces two double-strand breaks and deletes the intervening sequence. As with other prime editing tools, ...

  13. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... used in research have their genomes “gutted”, meaning that all viral genes except ITRs (inverted terminal...capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be...

  14. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...Do I have to be on LinkedIn?” For anyone who is planning or might need to plan for a career outside academia...hiring managers will think it is odd. For those planning a career in academia, it may not be required, ...

  16. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interactions, Ramanathan et al. designed an RNA bait containing three well conserved but not well characterized... and post-transcriptional regulation of SM1v1-containing RNAs could play a role in the severity of breast...

  17. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... two dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation) domains... psMEK2, psRaf1, and psCDK5. MEK2 is a serine/threonine kinase with a similar 3D structure to MEK1, but...

  18. Working with Nuclear Receptors

    Type
    Blog Post
    ...coregulator-related global gene profiling datasets in to our data mining tool, Transcriptomine. We are currently nearing...2001). Nuclear receptors and lipid physiology: opening the X-files. Science 294, 1866-1870. (PubMed) Lonard...
Showing: 701 - 720 of 975 results