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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,046 results for: ins;

Showing: 361 - 370 of 1046 results

  1. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...classified as a type V CRISPR system. Like Cas9, Cpf1 contains a RuvC-like endonuclease domain, but it lacks ...off-target editing efficiency. Kim et al. and Kleinstiver et al. characterized genome-wide editing efficiency... to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches...essential function in Cpf1-mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with ... are indicated below each graph. Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in ...activity than that previously observed with SpCas9. Kleinstiver et al. suggest that AsCpf1’s off-target rate ...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

  2. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...days inside the mother, the zebrafish is an ideal system to observe embryogenesis live. The origins, divisions...implemented in zebrafish. Developmental biology insights using lineage tracing in zebrafish To highlight...lineage tracing approaches that led to important insights in developmental biology. Microscopy-based lineage...example, neuronal activity can be tracked in whole brains of swimming, actively learning larvae, providing...resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these caveats, several...highlight how these advantages can translate into insights in developmental biology, and advances in this...zebrafish behavioral studies Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources...

  3. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...next-generation sequencing (NGS). However, Sanger sequencing remains useful for small sample sizes and shorter regions...occasionally still use Sanger sequencing to confirm an insert that is repetitive or otherwise hard to sequence...time-consuming, depending on the library prep and instrumentation used.  Here at Addgene, we sequence every plasmid...high-throughput compatibility. However, there are some instances where we use long-read sequencing or Sanger sequencing...DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the nanopore...nanopore, the electrical current recorded by the instrument changes. Base-calling software can then identify...SPAdes, Shovill, apc, and NOVOPlasty. The pipeline contains three de novo assemblers, so if one fails to generate...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    ... In this iteration, the size of the lentiviral insert was minimized to reduce recombination and avoid...transcriptase enzymes, inhibition of host cell proteins, and modifications to the pegRNA. The Britt Adamson...nucelocapsid protein, one of the virus’s most abundant proteins and a critical component for viral genome packaging...-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies...middle panel). Samples were also immunoblotted against vinculin as a loading control (right panel). Image... of ssAAV, a targeting sequence of miR124T was inserted into the vector to silence transgene expression...editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts ...

  5. Viral Production at Addgene

    Type
    Blog Post
    ...of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction of total...relative stoichiometric ratios of the viral capsid proteins VP1, VP2, and VP3. Samples of viral preparations...subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total.... Three to five days later, cell cultures are inspected for sterility. Transducibility Some viral vectors...virus from Addgene, read our detailed recipient instructions for next steps. Plan an experiment using our...plasmid in the viral preparation by performing PCR against unique regions of the transfer plasmid.   Lentivirus...

  6. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...form that can be understood by others, balanced against how often such data is likely to be accessed. Adding...not averse to making data available, but there remains concern about the extra work this may entail.  ...parsed by someone other than the actual author. Instead, selected images are said to be representative ...another issue of proprietary data formats. Many instruments store raw data in formats that require specific...supported/readable. Is the author or their host institution expected to provide data in formats exported ...integrate datasets with their resultant figures. Instead of providing a “dead end” figure or table, clicking...natural, albeit unintentional, tendency to work against transparency. This opens science to charges of ...

  7. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...cap: institutions of higher education nonprofit entities related to or affiliated with institutions of ...application on their behalf.  If an individual obtains a cap subject H-1B (last year less than 30% of ...exempt from the cap if the applicant works at institutions of higher education, a related or affiliated... lead role or critical role at a prestigious institution: This criterion is consistently under scrutiny...to self-petition. You need the support of your institute to do this one. Universities will usually only...professors. Casseus Law has seen that research institutions are willing to sponsor postdoctoral researchers...application is reserved for long term employees of the institute. For the EB-1B, you need to prove 3 threshold ...

  8. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the piñata effect ... group (Biology of Host-parasite Interactions, Institut Pasteur, Paris) that changed genome editing in... perspective, the Plasmodium falciparum genome contains only 0.66 million targetable NGG PAM sites whereas...and on-targeting likely more efficient.  These insights are hard to appreciate without computational support...each item changes from what the reviewers so painstakingly assessed to something a bit more representative...tools The goal of this post is to provide some insight into available CRISPR software tools, what problems... is already covered in the published articles. Instead, I have broken down the tools into the individual...

  9. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...that red fluorescent proteins tend to be less sensitive than green fluorescent proteins. nLightR did perform... corresponding pixel-wise ΔF/F0 heatmaps. White insets indicate surface expression of the indicators over...based on alpha-1a adrenergic receptor; nLightG contains circularly permuted green fluorescent protein ... alpha2-AR antagonist, nLight sensors are not. Instead, they are blocked by alpha1-AR antagonist. This...and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, ...More resources on the Addgene blog Fluorescent proteins 101: Fluorescent Biosensors Which Fluorescent ...

  10. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...NHEJ) pathway, leading to nonspecific nucleotide insertions, dele­tions or other mutations, referred to as...sin­gle-stranded oligodeoxynucleotide (ssODN), which contains the intended sequence changes to be incorporated...over 90% of the HDR edited alleles had unwanted insertions/deletions, therefore making those alleles useless... allele is accurately edited, the other likely contains unwanted indels. Following this observation, we...cut-to-mutation distance, the mutation may only be inserted at one of the alleles, but not the other – the...equally mixed with another ssODN template that contains the same CRISPR/Cas-blocking mutation but not ...currently a Professor for Neurobiology at the Institute for Stroke and Dementia Research, Ludwig-Maximilians-University...
Showing: 361 - 370 of 1046 results