We narrowed to 1,009 results for: RING
-
TypeBlog Post...acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...
-
Luminescent Imaging with Nano-lanterns
TypeBlog Post...to rely on differences in emission wavelength. Filtering the emission wavelength partially separates the... Mary Gearing... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared... -
Antibodies 101: ChIP
TypeBlog Post... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight...and the Three Steps (Crosslinking, Fragmenting, Capturing), if you ask me. Each of the three steps need... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...you are studying a protein that may bind a ligand during your experiment, then you’d want to make sure that... scientists can freeze hybridoma cells before culturing or they can clone the genes from the hybridoma... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...molecules to either activate or inhibit neuronal firing. As GPCRs are the largest class of signal transducing... and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids ... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post... change allowing high affinity binding to SspB, brings together the split botulinum neurotoxin halves ... to diffuse in and out but prohibit water from entering their hollow interiors. The Shapiro lab discovered... -
Plasmids 101: Inducible Promoters
TypeBlog Post...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light... Mary Gearing... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case... -
Plasmids 101: Yeast Vectors
TypeBlog Post...however, they tend to be unstable and may be lost during budding. Yeast Centromere plasmids (YCp): These...with yeast, due to high rates of spontaneously occurring resistant mutants and the insensitivity of yeast... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...informing potential for success in a patient-mutation bearing knock-in mouse model for CTD. Congratulations the...questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building upon... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether... and the concentration of the protein solution. During the experiment, the solution is diluted, complicating... -
Antibodies 101: Conjugation
TypeBlog Post...spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores... -
Antibodies 101: Affinity Reagents
TypeBlog Post...similar to a Fab fragment but created through engineering instead of cleavage. They are comprised of the...stable up to 80°C and can refold correctly after denaturing. Designed ankyrin repeat proteins, or DARPins... -
Transferable Skills Guide: Public Speaking
TypeBlog Post... could present something coherent or to quickly string together all the words that I could remember related...conferences, or even just provide quick updates during a company meeting increases visibility of your ... -
The time and cost required to make a plasmid
TypeBlog Post...them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...you on LinkedIn, you will lose opportunities and hiring managers will think it is odd. For those planning...any of these people, including and especially the hiring manager. This is considered intrusive and it’s ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity as a biological ...benthamiana or Arabidopsis thaliana (Xing et al., 2016)). During Agrobacterium-mediated DNA transfer, each GOI is... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post... a musician, then metastasize is touring. But you know what a touring musician needs? A backstage team... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...purification preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce...events that occur on a short timescale, such as during drug action or cell signaling. Limitations of RaPID...