We narrowed to 856 results for: HA

1. Plasmids 101: Origin of Replication

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   Blog Post

   ... proteins, respectively. A plasmid's copy number has to do with the balance between positive and negative...

2. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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   Blog Post

   ... interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation...

3. Plasmid Cloning by PCR

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   Blog Post

   ...plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...

4. Don’t Wait To Launch Your Health Science Startup

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   Blog Post

   ...this deter your ambition. If you believe your idea has potential to make a difference, then make sure you...

5. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   Blog Post

   ...Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now, one ...

6. Management for Scientists: Delegating is Key

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   Blog Post

   ...individual who is a local expert in that task (or has the bandwidth to become an expert) the end result...

7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   ...protein, if you know that your protein of interest has a similar spectrum. For example, here's a screenshot...

8. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

   ...team isn’t “one size fits all”. Each team member has unique motivations, strengths, and learning styles...

9. Antibodies 101: Flow Cytometry Controls

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   Blog Post

   ...setting up your initial voltages and gates. Ashlyn has a research background in CD8+ T cells and cancer ...

10. Antibodies 101: ChIP

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    Blog Post

    ...an antibody for the first time, look for one that has been validated in multiple assay types. Remember ...

11. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    Blog Post

    ...and its variants. Yet, the B.1.351 variant, which has only 9 different mutations compared to the wild-type...

12. New and Upcoming Viral Vectors - May 2020

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    Blog Post

    ...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...

13. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ...during the course of the study, say a fifth time has unpredictably high rates, should be reported as a...

14. The Importance of High Titer for AAV Transductions

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    Blog Post

    ...In order for an AAV particle to infect a cell, it has to come into physical contact with that cell. Since...

15. Plasmids 101: Inducible Promoters

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    Blog Post

    ...reporter gene transcription to take place. Addgene also has popular light-regulated two component systems designed...

16. Addgene Depositors Get More Citations

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    Blog Post

    ...associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these plasmids. While...

17. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post

    ...internal ribosomal entry site and mCherry. If a variant has wild-type levels of abundance, the eGFP will be stable...

18. K. phaffii: Rising to the Occasion

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    Blog Post

    ...proliferation. Genome manipulation K. phaffii’s genome has been sequenced for some time, clearing the greatest...

19. How Addgene Creates Content

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    Blog Post

    ... updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. ...

20. Summer SciComm Series: A PhD in Science Communication

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    Blog Post

    ...with chemicals in it,” leaving me to wonder how he has survived this long without downing any dihydrogen...