We narrowed to 765 results for: Mos

1. Droplet Digital PCR for AAV Quantitation

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   ...virus in reaction mix D = Dilution factor of virus Most droplet readers have a few channels for detecting...

2. MXS Chaining

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   ...because CpG dinucleotides are underrepresented in most vertebrate genomes (Josse et al., 1961, Swartz et...

3. Plasmids 101: Colony PCR

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   ...Designing colony PCR primers The first and perhaps most important step to colony PCR is designing primers...

4. Tips for Screening with Yeast Two Hybrid Systems

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   ...will increase the stringency of your binding. And most importantly: Independently validate identified binding...

5. Cpf1: A New Tool for CRISPR Genome Editing

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   ...does not mediate detectable off-target cleavage. Most gRNAs directed low-frequency Cpf1 cleavage at 1-...

6. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   ... different cells than were originally requested. Most of our samples (98.2%) transform the first time ...

7. A Career in Grants: How To Become a Grant Professional

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   ...career path.  A part of the career path story for most established grant professionals is learning to succeed...

8. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...Institute) has consistently been one of the repository’s most frequently requested plasmids. I wondered how many...

9. Antibodies 101: The Four ELISAs and When to Use Them

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   ...source of cross-reactivity.  Sandwich ELISAs In the most common type of ELISA, the sandwich ELISA, users ...

10. Hot Plasmids Spring 2024

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    ...SARS-CoV-2 nucelocapsid protein, one of the virus’s most abundant proteins and a critical component for viral...

11. Plasmids 101: Broad Host Range Plasmids

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    ... plasmids all replicate in E. coli, which is the most commonly used bacteria in lab manipulations. Table...

12. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    ...high concentration of FLEx vectors guarantees that most cells are transfected with all labeling plasmids...

13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...directed repair, since HDR-mediated gene editing is most efficient when target sites are located in close...

14. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...proteins, finding that APOBEC3A and APOBEC3bctd are the most efficient (Martin et al., 2019). TLDR: The eGFP ...

15. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ...first try to produce GVs in their own lab using the most reliable method, which is the transient cotransfection...

16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...compatible PAM at your site of interest, that is the most efficient option. If there is no NGG in your genomic...

17. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...studies have been essential for this task. However, most lesioning methods destroy areas larger than intended...

18. Save Time with Transient Plant Leaf Transformations

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    ...tissue (see Figure 3). This method is probably the most scalable method. Transfer your plant into the dark...

19. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...multiplex sample, the cut site will be aligned to the most 5’ cut site.     Indel Distribution In the Indel...

20. Transferable Skills Guide: External Collaborations

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    ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship...