We narrowed to 772 results for: Mos

1. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   ...cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker for IF. However, you ...

2. Course-Based Undergraduate Research Experiences (CUREs)

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   ...), decided to take a different approach. His own most impactful learning experiences happened in the lab...

3. CRISPR 101: Making a Knock-In Cell Line

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   ... other edits Like I said earlier, HDR is not the most common editing pathway for DNA repair. To tilt the...

4. Droplet Digital PCR for AAV Quantitation

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   ...virus in reaction mix D = Dilution factor of virus Most droplet readers have a few channels for detecting...

5. Tips for arabidopsis transformation

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   ... region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate to begin working...

6. Simplify Cloning with in vivo Assembly

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   ...common lab cloning strain has failed so far. What is most important is the transformation efficiency of the...

7. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...overexpression library identified several overlapping genes, most genes were unique to the individual libraries. These...

8. Plasmids 101: Colony PCR

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   ...Designing colony PCR primers The first and perhaps most important step to colony PCR is designing primers...

9. Tips for Screening with Yeast Two Hybrid Systems

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   ...will increase the stringency of your binding. And most importantly: Independently validate identified binding...

10. Cpf1: A New Tool for CRISPR Genome Editing

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    ...does not mediate detectable off-target cleavage. Most gRNAs directed low-frequency Cpf1 cleavage at 1-...

11. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ... different cells than were originally requested. Most of our samples (98.2%) transform the first time ...

12. A Career in Grants: How To Become a Grant Professional

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    ...career path.  A part of the career path story for most established grant professionals is learning to succeed...

13. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...multiplex sample, the cut site will be aligned to the most 5’ cut site.     Indel Distribution In the Indel...

14. Transferable Skills Guide: External Collaborations

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    ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship...

15. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...studies have been essential for this task. However, most lesioning methods destroy areas larger than intended...

16. Save Time with Transient Plant Leaf Transformations

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    ...tissue (see Figure 3). This method is probably the most scalable method. Transfer your plant into the dark...

17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...directed repair, since HDR-mediated gene editing is most efficient when target sites are located in close...

18. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...proteins, finding that APOBEC3A and APOBEC3bctd are the most efficient (Martin et al., 2019). TLDR: The eGFP ...

19. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post

    ...type of readout allows for nucleic acid detection almost anywhere as lateral flow strips are easy to transport...

20. Plasmids 101: Plasmid Incompatibility

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    ...same replication control machinery within the cell. Most plasmids encode a negative regulation system involving...