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We narrowed to 949 results for: ache

Showing: 721 - 740 of 949 results

  1. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...into the host cell’s genome and then use cell machinery for transcription and translation. Here, we will...Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated to produce...

  2. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...cells stay in an HDR-promoted cycle longer. This approach may have some negative outcomes on viability if... as 10 hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    ...Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....it into sticking to the hydrophobic glass filter. Each glass filter can capture up to 150 ug of DNA, and...

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...organization with a different person responsible for each figure. Others still are more casual with a single... applications and should be carefully chosen for each group and topic. For example, it may be useful to...

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    ...culture inoculated from a colony on a plate will reach an optimal density in 18-24 hours. I then add 0.5...very important to select multiple T1 plants from each transformation. I like to isolate a minimum of five...

  7. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ..., Zetsche et al. developed a Cpf1 multiplexing approach using a single crRNA array to express up to 4 ...LbCpf1 (blue). Mismatch positions are indicated below each graph. Figure from Kleinstiver et al., 2016. ...

  9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Although not listed here, many permutations within each color also exist with only slight variations separating...excitation/emission spectra are used; one fused to each protein or domain being tested. Find FRET plasmids...

  11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...and might open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents...found that downregulating expression of miR124a achieved a similar improvement in the forced swim test....

  13. Lab to Office Culture Shock

    Type
    Blog Post
    ...to office will likely happen eventually. If you approach that transition dreading the change, the adjustment...adjustment period will be that much longer. If you approach the transition embracing the opportunity for growth...

  14. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...efficient base rewriting in the genome became achievable in 2016 when two independent groups (Komor et...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or...

  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...is codon optimization? Codon optimization is an approach in gene engineering to improve gene expression... several codons that encode the same amino acid. Each organism, however, may preferentially use one codon...

  16. Hot Plasmids - August 2020

    Type
    Blog Post
    ... have created a yeast-based directed evolution approach with some notable improvements. Directed evolution...specific guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...combined with different fluorescent reporters for each enzyme. In addition, Csm6, a CRISPR type-III effector...Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti...

  18. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...measure the target. However, the two different approaches allow for optimized strategies for differing ...specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must be carefully...

  19. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...withstand ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar and Sandoval... can bind and transduce many cell types before reaching the target cells. VSV-G is also toxic at high ...

  20. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...re likely to find current job openings. A good approach is to do this first for companies at which you...originally called about, you can keep in touch and reach back out when you are looking to change positions...
Showing: 721 - 740 of 949 results