... postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters...peer review and grant awards systems need to be radically overhauled.  Finally, the science publishing system...Associate Professor of Systems Biology at Harvard Medical School, titled her presentation “How to Train Your...and take action. More Reading: “Rescuing US biomedical research from its systemic flaws” by Bruce Alberts...

...this area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... same Polish sub-dialect making their Skype communications extra fun) who shared first authorship on their...for billions of years (…) Bacteria are really sophisticated”.     New to CRISPR/Cas9 Technology? Learn ...

... Howarth’s lab at the University of Oxford is dedicated to generating new tools to manipulate biology ...able to grab on to proteins irreversibly in a genetically encoded way. SpyTag is nice and small, just 13... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the...this tool? Howarth: Polyproteins are useful for mechanical study of proteins by atomic force microscopy ...

...out that a PhD teaches analytical and critical thinking skills that are applicable to a variety of careers... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) who took the time...

...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...light. As the researchers described in Nature Communications last year, “The capability to control spectral...of specific wavelength and intensity allows for optical labeling and tracking of proteins, organelles, ...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670... are always looking to improve upon existing genetically-encoded probes by making them brighter, more ...

...genes are truly dispensable or are the current identification methods missing something? Here we will review...review some time-tested gene identification systems that may have a leg up on their modern counterparts...throughout an organism’s genome. These reporters have historically been used extensively in fly, mouse, and yeast.... These are effective tools but neglect some biologically relevant situations. Tissue-specific genes...considerable distance away from a gene which can make identification of the gene itself difficult. The best trap...

...whether a cell line is known to be genetically manipulated, its anatomical origin, if cell line has ever been...is resistant to specific compounds, links to publication references, and cross-references with cell line....  For those wanting to access Cellosaurus programatically, their API allows fine grained query and retrieval...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or...

...sequences To design experiments for further plasmid verification Identifying plasmid features While our quality...promoters, selectable markers, tags, origins of replication, and fluorescent proteins that are already included...reference sequences. Using Snapgene for additional verification experiments: Primer and restriction digest design...repetitive regions. These regions require additional verification experiments that can be partially designed using... plasmid verification...

...re proud to honor the creativity, passion, and dedication to sharing epitomized by the creators of these...plasmids through our repository since we know biological resource centers, including Addgene, positively...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...Since we so sincerely appreciate our depositor’s dedication to scientific sharing, we wanted to celebrate.... “Addgene has made it possible to eliminate geographical restrictions in life science research." Claim...

...demonstrate the most exciting application for ARG’s: in vivo imaging. Specifically, arg1 expressing E. coli... interpret signals from these reporters. Lack anatomical context/spatial localization: In vivo imaging...intestinal tract and has been used for 100 years to clinically treat enteric infection and inflammatory bowel...abdomen. arg1 signal, however, can be overlaid onto anatomical ultrasound imaging to show where the bacteria...

...be used to generate organoid cultures with a modification of interest. While the authors optimized the...mechanisms related to morphogenesis, cell fate specification, and human disease.     Sun, et al. bioRxiv...fluorescent biosensor based on the parasitic Striga hermonthica SL receptor HTL7. This novel type of biosensors...could be used in high throughput screening for agrochemical compounds that have the potential to regulate...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...

...protein transducers and that the potency and efficacy is typically non-uniform across the transducerome for...TRUPATH collection allows researchers to measure the physical association of combinations of different Gα, Gβ...well known and, as a result, a target of many pharmaceutical drugs, there is still only a partial understanding...Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the...

...All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's introduction. His willingness...Cas9 that is improving the nuclease specificity dramatically and reducing off-target effects. Other young...scientists discussed new CRISPR delivery systems, modifications to the RNA components, and challenging target...discussed some of the many challenges of creating biological circuits, and I can only say this science is ... deposited and distributed – have changed so dramatically in the past 4 years. I kept busy for the whole...

...while constant regions are identical for antibodies within certain classification groups called isotypes....published, knockout validated, and work in your application. But…they are the same isotype and therefore ...antibodies are plasmid-based and are created synthetically or derived from hybridoma antibody sequences...antibodies affects binding properties. In Journal of Biological Chemistry (Vol. 269, Issue 17, pp. 13048–13055...

...AAV viral preps to easily find and share useful technical data. These data focus on experimental design ...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...resource of over 100 entries is driven by the dedicated community of data-contributing researchers who...exactly which strain you used so if they wish to replicate your experiment, they know exactly where to get... we’d like to give a shoutout to all of those dedicated scientists who have contributed to the AAV Data...

...template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo ...differences in cleavage efficiency. If this finding is replicated in larger studies, it will represent a key advantage...site specified by the gDNA. Depending on your application, this could be favorable or unfavorable. In a...PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al.  “DNA-... PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute...

...culture), which is great for a wide variety of applications. But some techniques require you to select against...antibiotic. Successful editing of transformants then typically occurs in T0 plants (from callus) or T1 plants...cacti and beets. Betanin belongs to a different chemical family than the red/purple plant pigments found...these mutant plants. Using RUBY to simplify the identification of these mutants will speed up my work and ...student studying plant development and cell-cell communication in the Nimchuk Lab in the Biology Department...

...antibodies in your lab. Perhaps you’re even in that mythical place where antibodies are well-organized, documented... sample prep. This involves using heat and/or chemicals to break some of the bonds keeping the protein...epitope (right). Created withBioRender.com.   Biological Processes  If you’re feeling relieved that you...the available epitopes could be affected by, or critical to, the assay.   Next, look for antibodies that... also working for a Western because they both typically use denatured protein samples. If, however, you...

...analyzing the gene’s function. The process is shown graphically in the following figure, in which we add EcoRI...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...need to use the reverse complement to get PCR amplification. We can start similarly, taking the final 18...reaction using a kit, such as the QIAquick PCR Purification Kit. The PCR product is now ready for restriction...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

... The conventional approach has typically been to either systematically delete/overexpress endogenous genes... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-...