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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,082 results for: ica;

Showing: 721 - 740 of 1082 results

  1. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...reason for that. Lately, significant advances in the understanding and application of CRISPR/Cas9 technology... by even splashier news: Jiahao Sha of Nanjing Medical University had successfully used the CRISPR/Cas9...caused by genetic abnormalities, targeted genetic modification in monkeys is invaluable for the generation ... Times feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking...

  2. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...for reference: Untergasser Lab Preparation of chemically competent cells (protocol) Preparation of electrocompetent...until we are ready to perform transformations. A typical 200mL bacterial culture produces 20mL of competent...protocols below: Untergasser Lab Transformation of chemically competent cells (protocol)  Transformation of...being performed at Addgene, this adds up to a significant time savings. You can also save yourself time...plasmid is introduced to bacteria, the bacteria is typically grown in liquid culture without antibiotics to...

  3. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...you’ve saved. Repurposing regulated medical waste Regulated medical waste (RMV), which we collect in those...companies to obtain these labels! Reusing plastic Historically, scientists washed and reused materials such...flow cytometers. These fluidic machines handle biological samples and are cleaned between each use. You...those red biohazard bags, typically is destined for a landfill. However, Triumvirate Environmental developed...

  4. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ... as these recent blog posts on prime editing, IgnaviCas9, and Nanoblades), but we hope that this blog ...engineered tRNA adenosine deaminase (TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called... toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing) mediates targeted...(A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting Cas13 (dCas13) with...

  5. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...Scientists series will focus on an aspect of communication especially important for a manager – getting... Creating a culture of excellent, effective communication between all members of a team. As described ...what is going on and can contribute. Members periodically break, go exploring outside the team, and bring... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... opinions in meetings, via email, at formal communications events or at informal gatherings, like at lunch...

  6. Science as Art

    Type
    Blog Post
    ...problem is that scientists sometimes forget how to communicate about their work with non-academic people.” Daniel...while - a tool to expand perception, which is ironically what science is about. For me it is essential... time, defend the thesis, etc. Photography is critically important in many fields of biology and I believe...appreciated by a wider audience for more than just its biological importance. More of Daniel's stunning work can... new method to explore the inner structures of delicate plants and other organisms without destroying ...

  7. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...coming together, we can create a significant impact on both clinical care and public health for this current...match up scientists across fields with different technical knowledge that could help fight COVID-19. They...funding and volunteer opportunities, protocols, publications, and more to accelerate the pace of research...is based in Canada, much of the information is applicable to researchers worldwide. “COVID-19 has spread...

  8. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...imaging method that uses iterative staining and chemical bleaching to allow for high-resolution imaging...not recommended reagents) along with information critical for the performance of a reagent, e.g., antigen... repository for reagents, protocols, panels, publications, software, and datasets. It’s also a great place... (2022). IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse...Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial...

  9. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ... postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters...peer review and grant awards systems need to be radically overhauled.  Finally, the science publishing system...Associate Professor of Systems Biology at Harvard Medical School, titled her presentation “How to Train Your...and take action. More Reading: “Rescuing US biomedical research from its systemic flaws” by Bruce Alberts...

  10. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...this area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... same Polish sub-dialect making their Skype communications extra fun) who shared first authorship on their...for billions of years (…) Bacteria are really sophisticated”.     New to CRISPR/Cas9 Technology? Learn ...

  11. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... Howarth’s lab at the University of Oxford is dedicated to generating new tools to manipulate biology ...able to grab on to proteins irreversibly in a genetically encoded way. SpyTag is nice and small, just 13... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the...this tool? Howarth: Polyproteins are useful for mechanical study of proteins by atomic force microscopy ...

  12. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...out that a PhD teaches analytical and critical thinking skills that are applicable to a variety of careers... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) who took the time...

  13. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...light. As the researchers described in Nature Communications last year, “The capability to control spectral...of specific wavelength and intensity allows for optical labeling and tracking of proteins, organelles, ...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670... are always looking to improve upon existing genetically-encoded probes by making them brighter, more ...

  14. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...demonstrate the most exciting application for ARG’s: in vivo imaging. Specifically, arg1 expressing E. coli... interpret signals from these reporters. Lack anatomical context/spatial localization: In vivo imaging...intestinal tract and has been used for 100 years to clinically treat enteric infection and inflammatory bowel...abdomen. arg1 signal, however, can be overlaid onto anatomical ultrasound imaging to show where the bacteria...

  15. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...sequences To design experiments for further plasmid verification Identifying plasmid features While our quality...promoters, selectable markers, tags, origins of replication, and fluorescent proteins that are already included...reference sequences. Using Snapgene for additional verification experiments: Primer and restriction digest design...repetitive regions. These regions require additional verification experiments that can be partially designed using... plasmid verification...

  16. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...protein transducers and that the potency and efficacy is typically non-uniform across the transducerome for...TRUPATH collection allows researchers to measure the physical association of combinations of different Gα, Gβ...well known and, as a result, a target of many pharmaceutical drugs, there is still only a partial understanding...Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the...

  17. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...be used to generate organoid cultures with a modification of interest. While the authors optimized the...mechanisms related to morphogenesis, cell fate specification, and human disease.     Sun, et al. bioRxiv...fluorescent biosensor based on the parasitic Striga hermonthica SL receptor HTL7. This novel type of biosensors...could be used in high throughput screening for agrochemical compounds that have the potential to regulate...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...

  18. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...genes are truly dispensable or are the current identification methods missing something? Here we will review...review some time-tested gene identification systems that may have a leg up on their modern counterparts...throughout an organism’s genome. These reporters have historically been used extensively in fly, mouse, and yeast.... These are effective tools but neglect some biologically relevant situations. Tissue-specific genes...considerable distance away from a gene which can make identification of the gene itself difficult. The best trap...

  19. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...whether a cell line is known to be genetically manipulated, its anatomical origin, if cell line has ever been...is resistant to specific compounds, links to publication references, and cross-references with cell line....  For those wanting to access Cellosaurus programatically, their API allows fine grained query and retrieval...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or...

  20. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...re proud to honor the creativity, passion, and dedication to sharing epitomized by the creators of these...plasmids through our repository since we know biological resource centers, including Addgene, positively...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...Since we so sincerely appreciate our depositor’s dedication to scientific sharing, we wanted to celebrate.... “Addgene has made it possible to eliminate geographical restrictions in life science research." Claim...
Showing: 721 - 740 of 1082 results