Skip to main content
Addgene
Showing: 721 - 740 of 928 results

  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...applications. Get More Cpf1 Background Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN ... What we still don't know about Cpf1 In their original paper characterizing Cpf1, Zetsche et al. suggested...

  4. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...enable us to measure these properties, and what fascinating experiments could push these tools even further... Förster Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation...

  5. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...conjugated to the enzyme horseradish peroxidase (HRP). Finally, the substrate of HRP is added to the wells. This... the amount of your protein of interest in the original sample. The competitive ELISA is best used for...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... DNA, an editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent protein...Ward lab created a tool-kit to expand upon these original tools that allows for both spatiotemporal protein...

  8. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... One way to triumph over your neighbors is to eliminate the competition, and Penicillium isn’t the only...exciting molecular technologies!  This post was originally written by Jessica Welch in January 2017 and ...

  9. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...opportunities available to me as a graduate student culminated in my founding a nonprofit called Central Indiana...skills, and meet new people. There were visiting seminar speakers each week, my department and graduate ...

  10. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... structure of a protein, based on its atomic coordinates. These wire models were useful in that they not...download a pdb file of the structure’s atomic coordinates, and “open” these structures with molecular viewing...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...affinity for specific cell and tissue types. The combination of serotypes and psuedotypes means you can customize...stably express Cas effectors in specific tissues, eliminating the need to deliver the Cas protein altogether...

  14. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...Scientific #1011-8410) is also placed in the hood. Finally, a sterile 2X DMEM solution (diluted from 10X and... backup replicates of each clone in case of contamination. We do not normally use antibiotics. Lastly,...

  20. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...particle: pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences...
Showing: 721 - 740 of 928 results