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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 760 results for: mpo

Showing: 721 - 740 of 760 results

  3. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... expressed and purified from E. coli, retains important functional properties, and is amenable to a wide...

  16. Molecular Biology Reference

    Type
    Guide
    ...Additional Resources Plasmids are one of the most important tools in molecular biology. They allow scientists...study and have led to the understanding of many important molecular pathways and diseases. Here we present... proteins and reside on chromosomes, which are composed of DNA. These findings culminated in the central...transcribed into non-coding RNA (ncRNA) and play important roles in cellular processes and gene regulation...enable the isolation and purification of cellular components, such as DNA, but in practice this isolation ...accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and guide RNAs that target...your plasmid over multiple generations. It is important to distinguish that the antibiotic resistance ...

  17. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and common uses....require different isoforms of these packaging components. Therefore, gamma-retroviruses may not be efficiently...production, and scientists have made changes to these components for increased safety when producing viral vectors...produce gamma-retroviral vectors, these required components have been separated into distinct plasmids for...For a summary of all gamma-retroviral plasmid components, see the Gamma-Retroviral Plasmid Elements table...containing the transfer, envelope, or packaging components. The transfer plasmid encodes the gene of interest...particle containing matrix, capsid, and nucleocapsid components. pol in trans Precursor protein containing reverse...

  18. CRISPR Guide

    Type
    Guide
    ...approach. Engineered CRISPR systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated...Cas enzyme). The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding...mechanisms of CRISPR and some of the ways the CRISPR components are leveraged to achieve specific goals, such...Cas9 fused to proteins that modulate HDR or NHEJ components, like miCas9 , Cas9-DN1S , or others, can increase...the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit experimentally...change. Modern base editors include several fused components that promote the intended repair pathway or inhibit...V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that form a complex...
Showing: 721 - 740 of 760 results