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  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... about a week. Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol describes...useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged CRISPR knock-in...

  4. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands...092902 Liu Y, Cao Z, Wang Y, et al. Genome-wide screening for functional long noncoding RNAs in human cells...

  5. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...with more stringent stripping methods. If you are planning to detect multiple proteins through several rounds...membrane once before any detection steps to avoid retaining residual antibodies. If you have already applied...

  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...spread out the risk for the project by initially designing and ordering several dozen versions of the toehold...genome as unique barcodes to identify samples containing the virus. Of the 48 sensors, targeting 24 regions...

  7. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...the post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger RNA ...Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts ...

  9. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ... Epigenetic modifications play a large role in turning genes ‘off’ and ‘on’, signaling which genes should...methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase identification, and bisulfite...

  10. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...relatively cheap). Several protocols have been written outlining the different steps and materials required for...University.  His interests include the role of learning and memory in opioid addiction and science communication...

  12. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post
    ... the packaging cell genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005... also be aligned to plasmids in our inventory containing the suspected contaminating sequence. In most...

  13. The Scientific Conference Poster Session: Tips for Success

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    Blog Post
    ...success You’ve put a great deal of thought into designing your poster – now it’s time to practice your delivery... a list of posters at all? No problem! At the beginning of the session, take a lap around the posters ...

  14. Academic vs. Industry Postdocs

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    Blog Post
    ...publications, which may take a longer time to acquire. Learning about drug discovery: Unless your academic lab...positions all at once at the end of February or beginning of March, and the jobs are mixed into the general...

  16. PCR Applications for Copy Number Variation Assays

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    Blog Post
    ...negative samples and, if unexpected, verified by running the product on a gel. And only ddPCR provides absolute... primer  Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll...

  17. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... and the team set up a directed evolution and screening experiment with three of the proteins that had...several rounds of semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no...

  18. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...groups: voltage sensitive domains and Rhodopsin containing GEVIs. Chemigenetic sensors are better classified...two-part fluorescent system, and rhodopsin still functioning as the voltage sensor. FP-rhodopsin GEVIs have...
Showing: 721 - 740 of 975 results