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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,045 results for: ins;

Showing: 371 - 380 of 1045 results

  1. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...For example, if the DNA you’re inserting into the plasmid backbone contains a kanamycin resistance cassette...your inserted DNA) and further screening will help you determine if the plasmid backbone contains your...Selection is straightforward in cases where the inserted DNA includes an antibiotic resistance marker. ... the critical amino acid). Many common E. coli strains used for cloning are auxotrophic for specific amino... found on the original plasmid and either the insertion of a DNA fragment within the gene or loss of the...

  2. Hot Plasmids - October 2022

    Type
    Blog Post
    ... of light-gated potassium channelrhodopsins, named Kalium channelrhodopsins (KCRs), from a fungus-like...-2P Novel class of light-gated potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available...   Novel class of light-gated potassium channelrhodopsins by: Mike Lacy New work from the labs of Mingshan... attempts to engineer potassium-selective channelrhodopsins. HcKCR1 has a maximum absorption of 540 nm...plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins are natural light-gated potassium channels...than 50 antibodies target neuroscience-related proteins, but we recently added several broadly useful ...

  3. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...Cas as a powerful and adaptive defense strategy against life-threatening viruses. Evolving to survive ...clustered repeat sequences operated as a safeguard against bacteriophages did not come to light until 2007...re-exposure (2, 3). More specifically, specialized Cas proteins snip the foreign DNA into small fragments approximately...paste them into the CRISPR sequence. Separate Cas proteins then express and process the CRISPR loci to generate...out these functions using slightly different Cas proteins and PAM sequences. A total of eight evolutionarily...Cas systems provides powerful lines of defense against invading phages. Rising to the challenge - Evolution...Genome engineering and future directions CRISPR/Cas inspired genome editing was initially described by Cong...

  4. RUBY-Red Siliques

    Type
    Blog Post
    ...techniques require you to select against the presence of a transgene instead, which can’t be done if your ...antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually involves...are currently used in these situations to select against CRISPR positive plants, but screening these can...purple plant pigments found in all other plants, anthocyanins.   Figure 1: An adult plant expressing...different transformation events likely due to insertional effects in the genome. (Bottom panel, right) ... editing, then green offspring can be selected instead of red ones. Other innovative uses of RUBY are ...

  5. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...lab and Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical abstract, it’s a musical abstract...turns expression on and off with blue light.   Inspired by @UriAlonWeizmann, here my song about our paper...applications.   Figure 1: With blue light, the VVD domains of the BLADE dimerize and activates transcription...This construct, built from the pBAD33 backbone, contains BLADE, the PBAD promoter, and a mCherry reporter...your gene of interest. pBLADEONLY: This plasmid contains only BLADE and is based on the pTRC99a plasmid...transformed with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter. They created...Engineering AraC to make it responsive to light instead of arabinose. Nat Chem Biol. https://doi.org/10.1038...

  6. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...which contains the structural and packaging genes, the adenoviral helper plasmid which contains the proteins... to replicate,  and the transfer plasmid which contains the viral genome. In today’s blog post, we’ll...palindromic nature, and high GC content. This instability can cause partial loss of an ITR during routine...constructs in recombination-deficient bacterial strains, such as Stbl3s. ITR loss can be detected by restriction...include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of ...

  7. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...from Erik Snapp, Associate Professor at Albert Einstein College of Medicine, to learn how problematic ...Research Campus reminds us that, although fluorescent proteins are incredibly useful, chemical fluorophores can...Zika virus and, upon binding, produce reporter proteins. These toehold switches can be used to quickly...and Alexander Green (both formerly at the Wyss Institute) to learn how these amazing tools may be used ...different pH’s and some that are fused to fluorescent proteins. She also showed that R-bodies can rupture E. ... but, even with CRISPR, it isn’t always easy to insert a section of DNA into the genome of your target...Casilio, a system that uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety...

  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...comprised of two tandem fluorescent proteins: a relatively pH-insensitive RFP variant, DsRed.T3, that’s connected...biosensor contains mRFP and GFP fused to the autophagy-related microtubule-associated proteins 1A/1B light...lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for studying...the absence of autophagy, the untargeted Rosella remains in the neutral pH cytoplasm where it emits red ...PE due to a C-terminal glycine deletion, so it remains in the cytosol acting as an internal control. Autophagic...

  9. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ... well characterised antibodies against all human extracellular proteins with community-based validation...18:45 - Reagent Development - Steven C. Almo, Institute for Protein Innovation  1:18:45 - 2:01:18 (end...have a reproducibility “crisis” on our hands and instead provide a brief conceptual framework for the causes...ll see in Steve Almo’s talk about work at the Institute for Protein Innovation (IPI) (49:45 - 1:18:45)...while FPbase curates information about fluorescent proteins so you can best apply these technologies to your...formal systems, individual labs can certainly institute better experimental design practices including...Scientific Figures into Dashboards and Share Your Insights Who gives a tweet? 9 facts about scientists on...

  10. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning...into the mouse zygote (Qin et al., 2015).  When inside the zygotes, the gRNA will seek out its target ...pathway (HDR), be it a single nucleotide change, insertion of a reporter gene, or replacement of the murine...case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA locus as assessed by Southern blot...conventional methods. First, one can work with almost any strains of mice, as compared with conventional gene targeting...targeting, which is limited to a few strains, including 129 and C57BL/6, for which we have germline competent...with its human ortholog (greater than 5 kb), it remains to be seen whether CRISPR is as robust as conventional...
Showing: 371 - 380 of 1045 results