We narrowed to 1,009 results for: RING
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TypeBlog Post... could present something coherent or to quickly string together all the words that I could remember related...conferences, or even just provide quick updates during a company meeting increases visibility of your ...
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The time and cost required to make a plasmid
TypeBlog Post...them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources... -
Plasmids 101: Cre-lox
TypeBlog Post...enough that there is virtually no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate...are active only in certain cell or tissue types, during certain stages of development, or by making the... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...Nanoblades editing efficiencies of primary cells. Comparing Nanoblades to Existing CRISPR Delivery Methods... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...might consider prescribing GdnHCl to patients suffering from rare diseases arising from Arg mutations.... -
Rabies and Neuronal Tracing
TypeBlog Post...specific locations and give rise to progenitors during growth and development or that blood flows through.... While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency in a human cell...and ABE7.10. They quantified genomic editing by measuring the reduction in protein expression of these six... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...options are available to you, it may be worth considering an antibody raised in a different species. ... strategically plan primary/secondary antibody pairing such that each target has a unique species or isotype... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...hold great promise for improving or potentially curing human diseases that were previously thought to ...as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials, wild type AAV... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post... of Research (FoR) symposia held across the USA during this time have revealed that young scientists are...Biochemistry and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments... -
Plasmids 101: FLEx Vectors
TypeBlog Post...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...Addgene Blog Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...change (facilitated by the flexible linker) that brings the fluorescent units of the FRET pair together... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...their mental model of the protein. At some point during this interrogation process, they will almost invariably...recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target DNA strand... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post... use in the protocol, which cover 3 naturally-occurring nanobody structures (concave, loop, and convex...translated protein (sybody), ribosome, and mRNA. During the screening process, displayed sybodies that ... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...expression baseline. Copy number is then determined by comparing results to a standard curve. For CNV assays, it...gives an absolute value of DNA concentration. By comparing the number of positive droplets for the gene of... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...downstream flanking sequences to their input string (Figure 2). This string can then be used as input for the prediction... -
Hot Plasmids: Winter 2025
TypeBlog Post...to dicot species. In addition, they generated offspring tomato plants that inherited the genomic edit ...If they do (or if they don't!), please consider sharing your experience with other researchers by contributing... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...