We narrowed to 856 results for: HA

1. Antibodies 101: Flow Cytometry Controls

   Type

   Blog Post

   ...setting up your initial voltages and gates. Ashlyn has a research background in CD8+ T cells and cancer ...

2. How Addgene Creates Content

   Type

   Blog Post

   ... updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. ...

3. Finding and Joining Your Dream Lab

   Type

   Blog Post

   ...author plus the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

4. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   ... complement the one assay per gene approach that has been the historical standard. Specifically, in our...

5. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ...as an emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays ...

6. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   ...dual-vector system as opposed to a single-vector system) has several benefits. First, the low viral titers typically...

7. Summer SciComm: Preprints

   Type

   Blog Post

   ...review is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly...

8. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   ...conjugate on the antibody left exposed to light?  Has this antibody been left out, gone through a freeze-thaw-freeze...

9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   ...experiment check out Addgene’s protocol page, which has both protocols and videos for techniques in basic...

10. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    ...workshops and lessons learned: Since early 2018 the team has grown to include experts who could help provide new...

11. Viral Vectors 101: Biosensors

    Type

    Blog Post

    ...tools). It’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one...

12. GCE4All: Making Genetic Code Expansion Accessible

    Type

    Blog Post

    ...protocol to work. Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated...

13. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    ...particles, the functional infection unit. Each particle has a viral envelope, which fuses with the target cell...

14. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    ...temporary repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn...

15. OCaMP: A New Calcium Indicator for Neural Imaging

    Type

    Blog Post

    ...pAAV-CMV-OCaMP-WPRE pAAV CMV The Lohman lab has a limited quantity of AAVs that can be requested ...

16. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    ...repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity...

17. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    ...the most widely used CRISPR nuclease, recent work has shown that alternative enzymes may better suit specific...

18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    ...since its development, CRISPR/Cas9 genome editing has been used to study the effect of gene knockout in...

19. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    ...Collin’s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and ...

20. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    ...senior mentors available to go around. My advice has been to find 5 interested peers and form your own...