We narrowed to 852 results for: Met

1. RANbodies: Reporter Nanobody Fusions

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   ...Domain RANbody Reporters. Reporter Detection Method Uses Pros Cons Antigens HRP (P) Tyramide signal...

2. dTAG - You're it!

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   ... use of these plasmids are available here. The Methods section of Nabet et al., Nature Chemical Biology...

3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ... 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine anion-conducting channelrhodopsin...

4. All in a Twist: dsRNA

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   ...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...

5. CRISPR 101: Cas9 vs. The Other Cas(s)

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   ...from Cas9 and almost all other Cas enzymes is its method of DNA cleavage. Instead of cleaving DNA at a defined...

6. Antibodies 101: Polyclonal Antibodies

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   ...Protein A or Protein G purification is one common method. These proteins bind the IgG heavy chain. Running...

7. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   ...multiple) genetic perturbations in single cells. Methods to scale up multiplexed CRISPR systems for high-throughput...

8. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ...cumbersome, can lower on-target efficiency, and sometimes even increase off-target effects, respectively...

9. New Tool for Lineage Tracing: The ClonTracer Library

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   ... of questions surrounding cancer development, metastasis, and drug resistance, Bhang says “the [drug] ...

10. A Look at Addgene's QC Process

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    ...the plasmid to stop functioning properly or be something completely different than what was originally ...

11. Making CRISPR Plasmids Using Fragmid

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    ...available destination vectors for various delivery methods, along with compatible inserts. All destination...

12. Lab Automation at Addgene

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    ...automate and optimize more protocols in the future - something that will be key for our future growth. One of...

13. Adapting Toehold Switches to Detect Zika Virus

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    ...the Biodesign Center for Molecular Design and Biomimetics and the School of Molecular Sciences at Arizona...

14. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ... with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First, they...

15. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...al. Production of lentiviral vectors. Mol Ther Methods Clin Dev. 2016. PubMed PMID: 27110581. PubMed Central...

16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ... may enter “donor template vector”. Cloning Method Choose from: Gateway cloning, gene synthesis,...

17. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...chromosomes recombine during meiosis, creating gametes that contain a mix of genetic material from each...

18. Hot Plasmids and Viral Preps - January 2021

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    ...to date By Justin Ng High-throughput screening methods, such as fluorescence-activated cell sorting (FACS...

19. Hot Plasmids - October 2020

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    ...greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence...

20. Corralling and Cornering the Goldilocks Antibody

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    ...engineered.   Fig. 1: Antibody production methods    NeuroMab and others have an extensive library...