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We narrowed to 949 results for: ache

Showing: 741 - 760 of 949 results

  1. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ... filtered out inflammatory questions, and even coached us on how to write our AMA submission to avoid ...etc. database. Still have questions? I am easy to reach and happy to help. Additional Resources on the ...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...cells was being explored and it allowed control over each individual component of the system. However, it ...details on what exactly has been inserted or deleted. Each lab will use a slightly different method to genotype...

  3. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...because the purified plasmid, cells, and media used in each of the wells are not independent: they were derived...consuming and ultimately more important. Ideally, each biological replicate should use fresh media, test...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...however, eukaryotes have muliple polymerases which are each responsible for a specific subset of RNAs. To gain... in your host organism. Because transcription machinery differs between cell types or organisms, promoters...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...recombination events, but because these events are rare, each requires selection. These two steps are (Figure...medicine and clinical infectious diseases at the Massachusetts General Hospital where he is now an attending...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... makes it a perfect candidate for introductory teaching and research environments (Idalia & Bernardo, ...scientists around the world worked to characterize each gene’s function. Some key genetics research with...

  8. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...getting dark and wintry with the end of year approaching. We’re always looking for something to brighten...screening platform developed here is also a promising approach for developing sensors for other targets. Figure...

  11. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... molecules. FRET sensors face challenges of photobleaching, autofluorescence, and, in the case of exciting...27966527. PubMed Central PMCID: PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform...

  12. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...different sets of bands on the gel corresponding to each type of multimer present in your sample. In these...26104708; PMCID: PMC4480603. Holovics HJ, He Y, Lacher NA, Ruesch MN. Capillary gel electrophoresis with...

  13. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... causing proteins to migrate out of the gel and attach to the membrane.  In a wet transfer system the ...Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender AR (2019) Antibody validation...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...dirty”, with multiple sequence traces overlapping each other! When the count was done, 75% of the mice ...Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-...

  15. Finding Your Perfect Job After University

    Type
    Blog Post
    ...rural health clinic in Ghana. My role involved teaching the nursing students and community health workers...edge over other graduates during the recession. As each advertised job received many applications, employers...

  16. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...members really should have been getting feedback from each other. Though meetings are helpful, they are certainly...lines of communication, but if teammates don’t trust each other, none of that matters. If you are on a team...

  17. Important Considerations When Using AAVs

    Type
    Blog Post
    ...resistant to alcohol-based disinfectants. A 10% bleach solution should be used to clean AAVs from workspaces... different tropisms but, as you can see, AAVs of each serotype can transduce multiple tissues. So how ...

  19. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...target site (Fig. 3B). The INTEGRATE system can achieve ~100% integration of DNA up to 10 kB in size in...in the lab (Sleeping Beauty, piggyBac, and Tol2), each of which has several different applications (mutagenesis...
Showing: 741 - 760 of 949 results