We narrowed to 830 results for: eed

1. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   ...created in about a month and a half, without the need for complicated cloning of homology arms. MMEJ...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...Flexible GS linkers may also work. You will also need to generate negative control constructs — more on...

3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   ... and stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus...

4. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   ... systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...

5. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   ...Bacteria: Table 2 also has a link to the plasmid you’ll need to express your nanobody of choice in bacteria. ...

6. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   ...transgene into a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB...

7. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

   Type

   Blog Post

   ...promoter sequence. Thus, T7 RNA polymerase also needs to be present in the cells to transcribe the viral...

8. The Importance of a Fun Workplace: Company Culture at Addgene

   Type

   Blog Post

   ...sounds a little neurotic, but hey- sometimes fun needs to be had in an orderly and timely fashion. 9. ...

9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   ...this versatile protein to suit their experimental needs, and some of the common applications in the lab....

10. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    ...researchers can find the reagents and resources they need to cure Rett Syndrome.  If you’re new to Rett Syndrome...

11. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    ... a protein in its native host there is likely no need to codon optimize as this gene should already be...

12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    ... for some applications, it doesn’t eliminate the need to deliver larger cargo such as base editors and...

13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...requirement for Cas9-mediated DNA cleavage. The need for more PAM sequences While PAM sequences for the...

14. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    ...sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74(12), ...

15. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    ... (T) or cytosine (C). Several different teams succeeded with this approach around the same time (He et...

16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    ...arise and tools to deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish...

17. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components, ...

18. Viral Production at Addgene

    Type

    Blog Post

    ...) containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a ...

19. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    ...subsequent reprogramming success [1]. Fibroblasts need to be cultured and expanded in vitro for several...

20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type

    Blog Post

    ... and thus are deemed reliable, or if the sample needs to be quantified again. Some of the issues with...