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We narrowed to 1,082 results for: ica;

Showing: 741 - 760 of 1082 results

  1. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Blog Post
    ...All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's introduction. His willingness...Cas9 that is improving the nuclease specificity dramatically and reducing off-target effects. Other young...scientists discussed new CRISPR delivery systems, modifications to the RNA components, and challenging target...discussed some of the many challenges of creating biological circuits, and I can only say this science is ... deposited and distributed – have changed so dramatically in the past 4 years. I kept busy for the whole...

  2. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ...while constant regions are identical for antibodies within certain classification groups called isotypes....published, knockout validated, and work in your application. But…they are the same isotype and therefore ...antibodies are plasmid-based and are created synthetically or derived from hybridoma antibody sequences...antibodies affects binding properties. In Journal of Biological Chemistry (Vol. 269, Issue 17, pp. 13048–13055...

  3. RUBY-Red Siliques

    Type
    Blog Post
    ...culture), which is great for a wide variety of applications. But some techniques require you to select against...antibiotic. Successful editing of transformants then typically occurs in T0 plants (from callus) or T1 plants...cacti and beets. Betanin belongs to a different chemical family than the red/purple plant pigments found...these mutant plants. Using RUBY to simplify the identification of these mutants will speed up my work and ...student studying plant development and cell-cell communication in the Nimchuk Lab in the Biology Department...

  4. Antibodies 101: Epitope Availability

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    Blog Post
    ...antibodies in your lab. Perhaps you’re even in that mythical place where antibodies are well-organized, documented... sample prep. This involves using heat and/or chemicals to break some of the bonds keeping the protein...epitope (right). Created withBioRender.com.   Biological Processes  If you’re feeling relieved that you...the available epitopes could be affected by, or critical to, the assay.   Next, look for antibodies that... also working for a Western because they both typically use denatured protein samples. If, however, you...

  5. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post
    ...AAV viral preps to easily find and share useful technical data. These data focus on experimental design ...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...resource of over 100 entries is driven by the dedicated community of data-contributing researchers who...exactly which strain you used so if they wish to replicate your experiment, they know exactly where to get... we’d like to give a shoutout to all of those dedicated scientists who have contributed to the AAV Data...

  6. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo ...differences in cleavage efficiency. If this finding is replicated in larger studies, it will represent a key advantage...site specified by the gDNA. Depending on your application, this could be favorable or unfavorable. In a...PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al.  “DNA-... PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute...

  7. Plasmid Cloning by PCR

    Type
    Blog Post
    ...analyzing the gene’s function. The process is shown graphically in the following figure, in which we add EcoRI...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...need to use the reverse complement to get PCR amplification. We can start similarly, taking the final 18...reaction using a kit, such as the QIAquick PCR Purification Kit. The PCR product is now ready for restriction...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

  8. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ... The conventional approach has typically been to either systematically delete/overexpress endogenous genes... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-...

  9. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post
    ...imaging apoptosis in animals." Journal of the American Chemical Society (2019). PubMed PMID: 30821975. Additional...cell death" plays a pivotal role in an array of biological processes including development, the immune system...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters... reporter for imaging apoptosis in vivo." Cell chemical biology 23.7 (2016): 875-882. PubMed PMID: 27447051...

  10. Early Career Researcher Toolbox: Free Tools for Science Writing

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    Blog Post
    ... Writing schedule tools How to write a lot: A practical guide by Paula J. Silvia  This book has tips for...app lets you track habits, like writing. It’s basically the equivalent of crossing off days on the calendar...suggestions Hemmngway makes (it wasn’t designed for technical writing), it’s great at flagging passive voice...jargon, this tool can help you avoid overusing technical terms when writing for a general audience. The...goal of the phrase, such as being cautious, being critical, or defining terms. The phrases in Phrasebank ...

  11. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...backgrounds.” This work outlines the need for clear communication, fair credit through authorship, and acceptance...remotely. The authors stress the fact that “miscommunication, a lack of leadership, and inappropriate tools...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...setting. Check out the blog post “Fostering Communication in Remote Collaborations” to get more ideas....

  12. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...particle to infect a cell, it has to come into physical contact with that cell. Since there is a limited... by an anterograde AAV was shown to drop off drastically with lower titers. As you can see in figure 2...AAV-mediated gene expression in a target cell. Practical considerations If you have high quality AAV, you...Integration. Molecular therapy : the journal of the American Society of Gene Therapy. 2008;16(7):1189-1199.... Protocols for Work with Virus Watch Our AAV Purification Video ...

  13. Advice for Scientists Starting a Lab

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    Blog Post
    ... Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking the...environment for research is totally dependent on communication amongst the team so getting everyone talking...your department, and online. The Howard Hughes Medical Institute (HHMI) offers some great resources for...Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management for Postdocs and...

  14. Early Career Researcher Toolbox: Social Media for Scientists

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    Blog Post
    ...remotely as an Addgene Science Communication Intern. Developing science communication skills, while also learning...panel about “Social Media for Science Communication” at the American Society for Cell Biology (ASCB) 2018...Twitter Learn about using Reddit for science communication ...

  15. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the Allen ...directions. I would like to thank them for their time, dedication, advice and encouragement. Why virus? Why did...educational materials, and provides viral vector technical service (details about virus production protocols...word out about this new resource and providing fantastically useful educational content on viral vectors....the impression that it took a lot of work and dedication to do this well ... because it did. So finally...

  16. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ... the steps of gel purification and in vitro recombination. Across all modifications described in Trehan...effects of inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM...mutations, large deletions/insertions require complicated, often costly in vitro assembly methods. A new...recombination machinery, which facilitates easy genetic modification by homologous recombination. In most recombineering...

  17. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post
    ...activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or ... and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating...distinct effector domains that work together synergistically, modeled after natural transcription activation...demonstrated in a paper in Cell that they could typically achieve 90-99% knockdown with minimal off-target...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated...

  18. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post
    ...improves upon them. Methods for studying autophagy Biochemical assays Long-lived protein degradation: This...radioactivity in the culture supernatant (this is indicative of protein degradation). To control for protein...’s slow and only measures bulk autophagy. Morphological assays Electron microscopy: Electron microscopy...studying autophagy. This method relies on the identification of autophagic structures based on morphology...available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott, M., & Devenish...

  19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post
    ...time-consuming aspects of traditional cloning is the identification of clones that actually contain your insert...enzyme-mediated disruption of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful...ccdB and therefore would die. This procedure dramatically reduces the number of clones that do not contain...which can then be used for downstream cloning applications. While these two strains ultimately perform ...and ccdB Survival strains have been developed specifically with ccdB-containing vectors in mind, any plasmid...

  20. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ... year, narrow by article type (review, patent, clinical study, research article etc). There are also numerous...looking for a particular protocol or are trying to replicate an experiment, the methods section probably won...conclusions. In any case, the reader should give a critical eye to all results in the relation to how they...is science’s way of validating work prior to publication, it doesn’t mean that there are no mistakes or... biochemistry instructor in the Department of Biological Sciences at Louisiana State University. She is...
Showing: 741 - 760 of 1082 results