We narrowed to 939 results for: ina

1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...exchange occurs through two sequential homologous recombination events: The plasmid carrying the desired genetic...activity. ShadowR can be used as a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa ...

2. Experimenting with New Careers while in Grad School

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   ...opportunities available to me as a graduate student culminated in my founding a nonprofit called Central Indiana...skills, and meet new people. There were visiting seminar speakers each week, my department and graduate ...

3. Plasmids 101: Multicistronic Vectors

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   ... of two proteins from a single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes... Melina Fan...

4. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   ...the toolkit. We have included a wider range of terminators than are commonly used as we have tested them...start. They might want to use our promoters and terminators, but then they aren’t sure how to convert their...

5. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...these parts can be used over and over again in combination with novel genes to get very precise expression...clear, but the way I'd leave it is mostly to the imagination. The question is: what can you use biology to...

6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ..., such as knockout generation or homologous recombination.  However, experiments that require long-term... laboratory reagents, equipment, and expertise. Final thoughts The Cas9-gRNA RNP method provides scientists...

7. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ...possible, unlike methods involving homologous recombination. Additionally, these transposon also exhibit...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

8. Uncovering Ancestral Bias in CRISPR Research

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   ...actively use tools designed to help reduce or eliminate bias, just like the ones made available through.../s41586-022-04601-8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo,...

9. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant antibodies at Addgene...

10. Viral Vectors 101: The Retroviral Lifecycle

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    ...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...particle: pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences...

11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ... cognate protein. In some cases this property eliminates the need for removing excess dye from the sample..., are photoactivatable, and function in vivo.* Final thoughts The ease of use and continual improvement...

12. A Career in Grants: How To Become a Grant Professional

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    ...grant professional’s job duties can range from coordinating the grant application and submitting it, to ... with research grants? Are you interested in sustainable community development? Volunteer as a grant writer...

13. Negotiating Work and Life: How to Find the Joy

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    ...This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic... family member, want to train for a triathlon, finally buy that charming fixer-upper house you have always...

14. Save Time with Transient Plant Leaf Transformations

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    ...vinblastine and vincristine, are regulated in the medicinal plant, the Catharanthus roseus (Figure 1). The...antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-...

15. Plasmids 101: Codon usage bias

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    ...codons, the ribosome may therefore stall at inappropriate locations or fail to make it through the entire...and is folding properly. References 1. Angov, Evelina, et al. "Heterologous protein expression is enhanced...

16. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...of previously acquired skills such as speech, coordination, purposeful use of hands, and walking. They ...Miranda C, Dennys-Rivers C, Huffenberger A, Braun L, Rinaldi F, Wein N, Meyer KC, Solano S, Nguyen K , Lang ...

17. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination of many factors unique to this set of tools and...quickly. Beyond the service This brings us to the final piece of the viral vectors service puzzle: the usefulness...

18. Tips for Writing a Good Cover Letter

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    ...with qPCR would make it easy to master ddPCR.  Finally, it’s a good idea to state why you’re applying ... you're ready to go! Good luck! This post was originally written by Maria Soriano-Carot in September 2016...

19. Career Insights: Technical Support Specialist

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    ...team discussions can often lead to otherwise unobtainable solutions. To some extent there is the need ...role Talking to customers everyday, it can be fascinating and exciting to listen to the many individual...

20. CRISPR Activation: A Practical Guide

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    ...before you know your system is working as expected. Finally, for each gene you want to activate, you should...study in cis regulation and also suffer from an inability to easily deliver the appropriate isoform(s) of...