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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 985 results for: nin

Showing: 741 - 760 of 985 results

  1. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...publications, which may take a longer time to acquire. Learning about drug discovery: Unless your academic lab...positions all at once at the end of February or beginning of March, and the jobs are mixed into the general...

  5. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification... non-coding RNAs associated with telomeres by combining purification of a specific genomic region and ...

  7. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...  While their thorough analysis uncovered some concerning biases within widely used CRISPR screens, they...perpetuating racial bias. It is easy, even unintentionally, to attribute to an ancestry group what is ...

  8. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... expressing neurons. We tested four serotypes containing an expression cassette with an ubiquitous promoter...be cited, tracked, and appropriately credited, broadening the knowledge base of AAV vector performance ...

  9. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...the limitations to keep in mind whether you are planning your own ExM experiment or evaluating ExM images...expansion physically separates molecules while maintaining their relative positions, allowing you to resolve...

  10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...favorite protein and (2) a synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good...small-molecule labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free ...

  11. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...improve the frequency of HDR over non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter (Figure 2). The...transient leaf transformations are a great help in screening candidate genes and speeding up research. Certainly...

  13. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...that encode 20 amino acids and three stop codons, meaning that the same amino acid can be encoded by more...synonymous codons) may actually be critical for determining the rate of translation. The ribosome may slow...

  15. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...caught on quickly in the scientific community, turning slightly under ten requests a day in 2017 to over...repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1 are the...

  16. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ... coupled to the bacterial host's cell cycle, maintaining a stable concentration of plasmid. Similar to...levels low even if plasmid copy numbers are high, restraining further replication.   Figure 3: Comparison...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...their CROPseq method for optical pooled CRISPR screening, Paul Blainey’s lab has developed a multiplexing...resistant to viral transduction. Through in vivo screening of human promoters, the Zhang Lab found a short...

  18. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...are still many more features to consider when designing a full gene. A non-exhaustive list includes: ...interest codon optimized by ordering plasmids containing them from Addgene. If a plasmid at Addgene contains...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...work, a video abstract may be a useful tool for turning video views into online article downloads – especially...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...110.18 (2013). 7306-11. Pubmed PMID: 23589850. 9. Cronin, J., Zhang, X.Y., Reiser, J. “Altering the tropism...16101513. PubMed Central PMCID: PMC1368960. 10. Denning, W., Das, S., Guo, S., Xu, J., Kappes, J.C., Hel...
Showing: 741 - 760 of 985 results