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  1. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification... non-coding RNAs associated with telomeres by combining purification of a specific genomic region and ...

  3. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...the limitations to keep in mind whether you are planning your own ExM experiment or evaluating ExM images...expansion physically separates molecules while maintaining their relative positions, allowing you to resolve...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...  While their thorough analysis uncovered some concerning biases within widely used CRISPR screens, they...perpetuating racial bias. It is easy, even unintentionally, to attribute to an ancestry group what is ...

  5. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post
    ... expressing neurons. We tested four serotypes containing an expression cassette with an ubiquitous promoter...be cited, tracked, and appropriately credited, broadening the knowledge base of AAV vector performance ...

  6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post
    ...favorite protein and (2) a synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good...small-molecule labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free ...

  7. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...improve the frequency of HDR over non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the...

  9. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...work, a video abstract may be a useful tool for turning video views into online article downloads – especially...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...

  10. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ... gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter (Figure 2). The...transient leaf transformations are a great help in screening candidate genes and speeding up research. Certainly...

  11. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...that encode 20 amino acids and three stop codons, meaning that the same amino acid can be encoded by more...synonymous codons) may actually be critical for determining the rate of translation. The ribosome may slow...

  12. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...caught on quickly in the scientific community, turning slightly under ten requests a day in 2017 to over...repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1 are the...

  13. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ... coupled to the bacterial host's cell cycle, maintaining a stable concentration of plasmid. Similar to...levels low even if plasmid copy numbers are high, restraining further replication.   Figure 3: Comparison...

  14. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...their CROPseq method for optical pooled CRISPR screening, Paul Blainey’s lab has developed a multiplexing...resistant to viral transduction. Through in vivo screening of human promoters, the Zhang Lab found a short...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...are still many more features to consider when designing a full gene. A non-exhaustive list includes: ...interest codon optimized by ordering plasmids containing them from Addgene. If a plasmid at Addgene contains...

  16. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...110.18 (2013). 7306-11. Pubmed PMID: 23589850. 9. Cronin, J., Zhang, X.Y., Reiser, J. “Altering the tropism...16101513. PubMed Central PMCID: PMC1368960. 10. Denning, W., Das, S., Guo, S., Xu, J., Kappes, J.C., Hel...

  17. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...studies. These parameters are less important when designing a biosensor, as the distance between the two fluorophores...many measurement methods as feasible when first beginning to establish the FRET methodology for a given ...

  18. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...autofluorescence’ include riboflavin in the medium or flavin containing proteins in mammalian cells. Generally, autofluorescence...  References 1. Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane...

  19. Five Popular Model Organisms

    Type
    Blog Post
    ...before we even knew that DNA was genetic material (Jennings, 2011).   The fruit fly makes a great model organism...PMID: 23594743. PubMed Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology...

  20. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...specific elution of biotinylated proteins. For help determining which biotinylation reagent to use check out ...Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules on surfaces.” Journal...
Showing: 741 - 760 of 975 results